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Journal of Bacteriology, June 2007, p. 4187-4195, Vol. 189, No. 11
0021-9193/07/$08.00+0     doi:10.1128/JB.01712-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Pigment Analysis of "Candidatus Chlorothrix halophila," a Green Filamentous Anoxygenic Phototrophic Bacterium{triangledown}

Tien Le Olson,1 Allison M. L. van de Meene,1 J. Nicholas Francis,2 Beverly K. Pierson,2 and Robert E. Blankenship3*

Department of Chemistry and Biochemistry, Arizona State University, Tempe, Arizona 85287,1 Department of Biology, University of Puget Sound, Tacoma, Washington,2 Departments of Biology and Chemistry, Washington University, St. Louis, Missouri 631303

Received 6 November 2006/ Accepted 27 February 2007

The pigment composition of "Candidatus Chlorothrix halophila," a filamentous anoxygenic phototrophic bacterium found in Baja California Sur, Mexico, was determined. Previous work showed that bacteriochlorophyll c (BChl c) was the major pigment in "Ca. Chlorothrix halophila," but it was not clear if this bacterium also contains BChl a (J. A. Klappenbach and B. K. Pierson, Arch. Microbiol. 181:17-25, 2004). Here we show that in addition to BChl c, a small amount of a pigment that is spectrally indistinguishable from BChl a is present in cell extracts of "Ca. Chlorothrix halophila." Nevertheless, the BChl a-like pigment from "Ca. Chlorothrix halophila" has a different molecular weight and a different high-performance liquid chromatography elution time than BChl a from other photosynthetic bacteria. Based on mass spectrometry and other spectroscopic analysis, we determined that the BChl a-like pigment in "Ca. Chlorothrix halophila" contains a tetrahydrogeranylgeraniol tail rather than the phytol tail that is present in BChl a. The carotenoids and major BChl c homologs in "Ca. Chlorothrix halophila" were also identified. BChls c were found to be farnesol esterified and geranylgeraniol esterified.

* Corresponding author. Mailing address: Departments of Biology and Chemistry, Campus Box 1229, Washington University, St. Louis, MO 63130. Phone: (314) 935-7971. Fax: (314) 935-4432. E-mail: Blankenship{at}wustl.edu

{triangledown} Published ahead of print on 16 March 2007.

Journal of Bacteriology, June 2007, p. 4187-4195, Vol. 189, No. 11
0021-9193/07/$08.00+0     doi:10.1128/JB.01712-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • van de Meene, A. M. L., Olson, T. L., Collins, A. M., Blankenship, R. E. (2007). Initial Characterization of the Photosynthetic Apparatus of "Candidatus Chlorothrix halophila," a Filamentous, Anoxygenic Photoautotroph. J. Bacteriol. 189: 4196-4203 [Abstract] [Full Text]  


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