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Interspecies DNA Microarray Analysis Identifies WblA as a Pleiotropic Down-Regulator of Antibiotic Biosynthesis in Streptomyces ^,

Seung-Hoon Kang,^1, Jianqiang Huang,^2, Han-Na Lee,¹ Yoon-Ah Hur,¹ Stanley N. Cohen,^2* and Eung-Soo Kim^1*

Department of Biological Engineering, Inha University, Incheon 402-751, Korea,¹ Department of Genetics, Stanford University School of Medicine, Stanford, California 94305²

Received 27 November 2006/ Accepted 26 March 2007

Using Streptomyces coelicolor microarrays to discover regulators of gene expression in other Streptomyces species, we identified wblA, a whiB-like gene encoding a putative transcription factor, as a down-regulator of doxorubicin biosynthesis in Streptomyces peucetius. Further analysis revealed that wblA functions pleiotropically to control antibiotic production and morphological differentiation in streptomycetes. Our results reveal a novel biological role for wblA and show the utility of interspecies microarray analysis for the investigation of streptomycete gene expression.

Published ahead of print on 6 April 2007.

Supplemental material for this article may be found at http://jb.asm.org/.

These authors contributed equally to the work.