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Spontaneous trpY Mutants and Mutational Analysis of the TrpY Archaeal Transcription Regulator

L'ubomíra uboová, Kathleen Sandman, Elizabeth A. Karr, Andrew J. Cochran, and John N. Reeve^*

Department of Microbiology, Ohio State University, Columbus, Ohio 43210-1292

Received 31 January 2007/ Accepted 19 March 2007

Over 90% of Methanothermobacter thermautotrophicus mutants isolated as spontaneously resistant to 5-methyl tryptophan had mutations in trpY. Most were single-base-pair substitutions that identified separate DNA- and tryptophan-binding regions in TrpY. In vivo and in vitro studies revealed that DNA binding was sufficient for TrpY repression of trpY transcription but that TrpY must bind DNA and tryptophan to assemble a complex that represses trpEGCFBAD.

Published ahead of print on 30 March 2007.

Permanent address: Institute of Animal Biochemistry and Genetics, Ivanka Pri Dunaji, Slovak Republic.

This article has been cited by other articles:

• Karr, E. A., Sandman, K., Lurz, R., Reeve, J. N. (2008). TrpY Regulation of trpB2 Transcription in Methanothermobacter thermautotrophicus. J. Bacteriol. 190: 2637-2641 [Abstract] [Full Text]