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Journal of Bacteriology, July 2007, p. 4809-4814, Vol. 189, No. 13
0021-9193/07/$08.00+0     doi:10.1128/JB.01786-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

YbxF, a Protein Associated with Exponential-Phase Ribosomes in Bacillus subtilis{triangledown}

Ludek Sojka,1,2 Vladimír Fucík,1,3 Libor Krásny,1,2 Ivan Barvík,4 and Jirí Jonák1,2,5*

Department of Bacteriology, Institute of Molecular Genetics ASCR, Vídenská 1083, 142 20 Prague 4, Czech Republic,1 Laboratory of Molecular Genetics of Bacteria, Institute of Microbiology ASCR, Vídenská 1083, 142 20 Prague 4, Czech Republic,2 Department of Natural Compounds, Institute of Organic Chemistry and Biochemistry ASCR, Flemingovo nám. 2, 16610 Prague 6, Czech Republic,3 Institute of Physics, Faculty of Mathematics and Physics, Charles University, Ke Karlovu 5, 121 16 Prague 2, Czech Republic,4 Institute of Medical Biochemistry, First Medical Faculty, Charles University, Katerinská 32, 12108 Prague 2, Czech Republic5

Received 27 November 2006/ Accepted 20 April 2007

The ybxF gene is a member of the streptomycin operon in a wide range of gram-positive bacteria. In Bacillus subtilis, it codes for a small basic protein (82 amino acids, pI 9.51) of unknown function. We demonstrate that, in B. subtilis, YbxF localizes to the ribosome, primarily to the 50S subunit, with dependence on growth phase. Based on three-dimensional structures of YbxF generated by homology modeling, we identified helix 2 as important for the interaction with the ribosome. Subsequent mutational analysis of helix 2 revealed Lys24 as crucial for the interaction. Neither the B. subtilis ybxF gene nor its paralogue, the ymxC gene, is essential, as shown by probing {Delta}ybxF, {Delta}ymxC, or {Delta}ybxF {Delta}ymxC double deletion strains in several functional assays.

* Corresponding author. Mailing address: Institute of Molecular Genetics ASCR, Vídenská 1083, 142 20 Prague 4, Czech Republic. Phone: 420 241 063 273. Fax: 420 224 310 955. E-mail: jjon{at}img.cas.cz

{triangledown} Published ahead of print on 27 April 2007.

Journal of Bacteriology, July 2007, p. 4809-4814, Vol. 189, No. 13
0021-9193/07/$08.00+0     doi:10.1128/JB.01786-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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