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Journal of Bacteriology, July 2007, p. 4827-4836, Vol. 189, No. 13
0021-9193/07/$08.00+0     doi:10.1128/JB.00043-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Environmental Regulation of Pseudomonas aeruginosa PAO1 Las and Rhl Quorum-Sensing Systems{triangledown}

Kangmin Duan1,3 and Michael G. Surette1,2*

Department of Microbiology and Infectious Diseases,1 Biochemistry and Molecular Biology, University of Calgary, Calgary, Alberta, Canada T2N 4N1,2 Molecular Microbiology Laboratory, Faculty of Life Sciences, Northwest University, Xi'an 710069, China3

Received 9 January 2007/ Accepted 11 April 2007

The lasI-lasR and the rhlI-rhlR quorum-sensing systems in Pseudomonas aeruginosa regulate the expression of numerous cellular and secreted virulence factor genes and play important roles in the development of biofilms. The las and rhl systems themselves are known to be directly or indirectly regulated by a number of transcriptional regulators, and consequently, their expression is sensitive to environmental conditions. In this report, the activities of these two quorum-sensing systems have been examined systematically under 46 growth conditions, and the regulation by environmental conditions has been investigated. The relative timing and strength of expression of these two systems varied significantly under different conditions, which contrasts with the notion of a preset hierarchy with these two systems in P. aeruginosa. Depending on the growth conditions, the correlation between each synthase and its cognate transcriptional regulator also varied, suggesting that the transcription of these genes independently allows for further fine tuning of each system. Finally, we observe that the activities of both the lasI-lasR and the rhlI-rhlR quorum-sensing systems were dramatically enhanced in the presence of extracts of sputum samples from cystic fibrosis patients.

* Corresponding author. Mailing address: Faculty of Medicine, Department of Microbiology and Infectious Diseases, Biochemistry and Molecular Biology, University of Calgary, 3330 Hospital Dr. NW, Calgary T2N 4N1, Canada. Phone: (403) 220-2744. Fax: (403) 270-2772. E-mail: surette{at}ucalgary.ca

{triangledown} Published ahead of print on 20 April 2007.

Journal of Bacteriology, July 2007, p. 4827-4836, Vol. 189, No. 13
0021-9193/07/$08.00+0     doi:10.1128/JB.00043-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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