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Journal of Bacteriology, July 2007, p. 5034-5040, Vol. 189, No. 14
0021-9193/07/$08.00+0     doi:10.1128/JB.00317-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Octanoyl-Homoserine Lactone Is the Cognate Signal for Burkholderia mallei BmaR1-BmaI1 Quorum Sensing{triangledown}

Breck A. Duerkop,1 Ricky L. Ulrich,2 and E. Peter Greenberg1*

Department of Microbiology, University of Washington School of Medicine, Seattle, Washington 98195-7242,1 Bacteriology Division, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Maryland 217042

Received 3 March 2007/ Accepted 2 May 2007

Acyl-homoserine lactones (HSLs) serve as quorum-sensing signals for many Proteobacteria. Members of the LuxI family of signal generators catalyze the production of acyl-HSLs, which bind to a cognate receptor in the LuxR family of transcription factors. The obligate animal pathogen Burkholderia mallei produces several acyl-HSLs, and the B. mallei genome has four luxR and two luxI homologs, each of which has been established as a virulence factor. To begin to delineate the relevant acyl-HSL signals for B. mallei LuxR homologs, we analyzed the BmaR1-BmaI1 system. A comparison of acyl-HSL profiles from B. mallei ATCC 23344 and a B. mallei bmaI1 mutant indicates that octanoyl-HSL synthesis is BmaI1 dependent. Furthermore, octanoyl-HSL is the predominant acyl-HSL produced by BmaI1 in recombinant Escherichia coli. The synthesis of soluble BmaR1 in recombinant E. coli requires octanoyl-HSL or decanoyl-HSL. Insoluble aggregates of BmaR1 are produced in the presence of other acyl-HSLs and in the absence of acyl-HSLs. The bmaI1 promoter is activated by BmaR1 and octanoyl-HSL, and a 20-bp inverted repeat in the bmaI1 promoter is required for bmaI1 activation. Purified BmaR1 binds to this promoter region. These findings implicate octanoyl-HSL as the signal for BmaR1-BmaI1 quorum sensing and show that octanoyl-HSL and BmaR1 activate bmaI1 transcription.

* Corresponding author. Mailing address: Department of Microbiology, University of Washington School of Medicine, 1959 N. E. Pacific Street, Box 357242, Seattle, WA 98195-7242. Phone: (206) 616-2881. Fax: (206) 616-2968. E-mail: epgreen{at}u.washington.edu

{triangledown} Published ahead of print on 11 May 2007.

Journal of Bacteriology, July 2007, p. 5034-5040, Vol. 189, No. 14
0021-9193/07/$08.00+0     doi:10.1128/JB.00317-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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