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Journal of Bacteriology, July 2007, p. 5049-5059, Vol. 189, No. 14
0021-9193/07/$08.00+0     doi:10.1128/JB.00338-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Global Transcriptional Analysis of Streptococcus mutans Sugar Transporters Using Microarrays{triangledown} ,{dagger}

Dragana Ajdic* and Vi T. T. Pham

Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma 73104

Received 7 March 2007/ Accepted 4 May 2007

The transport of carbohydrates by Streptococcus mutans is accomplished by the phosphoenolpyruvate-phosphotransferase system (PTS) and ATP-binding cassette (ABC) transporters. To undertake a global transcriptional analysis of all S. mutans sugar transporters simultaneously, we used a whole-genome expression microarray. Global transcription profiles of S. mutans UA159 were determined for several monosaccharides (glucose, fructose, galactose, and mannose), disaccharides (sucrose, lactose, maltose, and trehalose), a ß-glucoside (cellobiose), oligosaccharides (raffinose, stachyose, and maltotriose), and a sugar alcohol (mannitol). The results revealed that PTSs were responsible for transport of monosaccharides, disaccharides, ß-glucosides, and sugar alcohol. Six PTSs were transcribed only if a specific sugar was present in the growth medium; thus, they were regulated at the transcriptional level. These included transporters for fructose, lactose, cellobiose, and trehalose and two transporters for mannitol. Three PTSs were repressed under all conditions tested. Interestingly, five PTSs were always highly expressed regardless of the sugar source used, presumably suggesting their availability for immediate uptake of most common dietary sugars (glucose, fructose, maltose, and sucrose). The ABC transporters were found to be specific for oligosaccharides, raffinose, stachyose, and isomaltosaccharides. Compared to the PTSs, the ABC transporters showed higher transcription under several tested conditions, suggesting that they might be transporting multiple substrates.

* Corresponding author. Mailing address: University of Oklahoma Health Sciences Center, Department of Microbiology and Immunology, 940 S. L. Young Blvd., Oklahoma City, OK 73104. Phone: (405) 271-1202. Fax: (405) 271-3117. E-mail: dragana-ajdic{at}ouhsc.edu

{triangledown} Published ahead of print on 11 May 2007.

{dagger} Supplemental material for this article may be found at http://jb.asm.org/.

Journal of Bacteriology, July 2007, p. 5049-5059, Vol. 189, No. 14
0021-9193/07/$08.00+0     doi:10.1128/JB.00338-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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