| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
,
Unité des Membranes Bactériennes, Département de Microbiologie, CNRS URA2172,1 Unité de Résonance Magnétique Nucléaire des Biomolécules, Département de Biologie Structurale, CNRS URA 2185, Institut Pasteur, 25-28 rue du Dr. Roux, 75724 Paris Cedex 15, France2
Received 14 February 2007/ Accepted 24 April 2007
On the basis of the three-dimensional model of the heme/hemophore TonB-dependent outer membrane receptor HasR, mutants with six-residue deletions in the 11 putative extracellular loops were generated. Although all mutants continued to be active TonB-dependent heme transporters, mutations in three loops abolished hemophore HasA binding both in vivo and in vitro.
Published ahead of print on 4 May 2007.
Supplemental material for this article may be found at http://jb.asm.org/.
| Appl. Environ. Microbiol. | Infect. Immun. | Eukaryot. Cell |
|---|---|---|
| Mol. Cell. Biol. | J. Virol. | Microbiol. Mol. Biol. Rev. |
| ALL ASM JOURNALS |