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Journal of Bacteriology, July 2007, p. 5379-5382, Vol. 189, No. 14
0021-9193/07/$08.00+0     doi:10.1128/JB.00251-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Mutagenesis and Molecular Modeling Reveal Three Key Extracellular Loops of the Membrane Receptor HasR That Are Involved in Hemophore HasA Binding{triangledown} ,{dagger}

Clément Barjon,1 Karine Wecker,2 Nadia Izadi-Pruneyre,2 and Philippe Delepelaire1*

Unité des Membranes Bactériennes, Département de Microbiologie, CNRS URA2172,1 Unité de Résonance Magnétique Nucléaire des Biomolécules, Département de Biologie Structurale, CNRS URA 2185, Institut Pasteur, 25-28 rue du Dr. Roux, 75724 Paris Cedex 15, France2

Received 14 February 2007/ Accepted 24 April 2007

On the basis of the three-dimensional model of the heme/hemophore TonB-dependent outer membrane receptor HasR, mutants with six-residue deletions in the 11 putative extracellular loops were generated. Although all mutants continued to be active TonB-dependent heme transporters, mutations in three loops abolished hemophore HasA binding both in vivo and in vitro.

* Corresponding author. Mailing address: Unité des Membranes Bactériennes, Département de Microbiologie, CNRS URA2172, Institut Pasteur, 25-28 rue du Dr. Roux, 75724 Paris Cedex 15, France. Phone: (33) 1 40 61 32 76. Fax: (33) 1 45 68 87 90. E-mail: pdelep{at}pasteur.fr

{triangledown} Published ahead of print on 4 May 2007.

{dagger} Supplemental material for this article may be found at http://jb.asm.org/.

Journal of Bacteriology, July 2007, p. 5379-5382, Vol. 189, No. 14
0021-9193/07/$08.00+0     doi:10.1128/JB.00251-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Krieg, S., Huche, F., Diederichs, K., Izadi-Pruneyre, N., Lecroisey, A., Wandersman, C., Delepelaire, P., Welte, W. (2009). Heme uptake across the outer membrane as revealed by crystal structures of the receptor-hemophore complex. Proc. Natl. Acad. Sci. USA 106: 1045-1050 [Abstract] [Full Text]  


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