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Journal of Bacteriology, July 2007, p. 5383-5386, Vol. 189, No. 14
0021-9193/07/$08.00+0     doi:10.1128/JB.00137-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Quorum-Sensing Regulation of the Biofilm Matrix Genes (pel) of Pseudomonas aeruginosa{triangledown}

Yumiko Sakuragi{dagger} and Roberto Kolter*

Department of Microbiology and Molecular Genetics, Harvard Medical School, 200 Longwood Avenue, Boston, Massachusetts 02115

Received 27 January 2007/ Accepted 2 May 2007

Quorum sensing (QS) has been previously shown to play an important role in the development of Pseudomonas aeruginosa biofilms (D. G. Davies et al., Science 280:295-298, 1998). Although QS regulation of swarming and DNA release has been shown to play important roles in biofilm development, regulation of genes directly involved in biosynthesis of biofilm matrix has not been described. Here, transcription of the pel operon, essential for the production of a glucose-rich matrix exopolysaccharide, is shown to be greatly reduced in lasI and rhlI mutants. Chemical complementation of the lasI mutant with 3-oxo-dodecanoyl homoserine lactone restores pel transcription to the wild-type level and biofilm formation ability. These findings thus connect QS signaling and transcription of genes responsible for biofilm matrix biosynthesis.

* Corresponding author. Mailing address: Department of Microbiology and Molecular Genetics, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115. Phone: (617) 432-1776. Fax: (617) 738-7664. E-mail: rkolter{at}hms.harvard.edu

{triangledown} Published ahead of print on 11 May 2007.

{dagger} Present address: Faculty of Life Sciences, Department of Plant Biology, University of Copenhagen, Frederiksberg DK-1871, Denmark.

Journal of Bacteriology, July 2007, p. 5383-5386, Vol. 189, No. 14
0021-9193/07/$08.00+0     doi:10.1128/JB.00137-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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