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Journal of Bacteriology, August 2007, p. 5782-5786, Vol. 189, No. 15
0021-9193/07/$08.00+0     doi:10.1128/JB.00411-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Double-Strand Break Generation under Deoxyribonucleotide Starvation in Escherichia coli{triangledown}

Estrella Guarino, Israel Salguero, Alfonso Jiménez-Sánchez, and Elena C. Guzmán*

Departamento de Bioquímica, Biología Molecular y Genética, Facultad de Ciencias, Universidad de Extremadura, 06080-Badajoz, Spain

Received 21 March 2007/ Accepted 18 May 2007

Stalled replication forks produced by three different ways of depleting deoxynucleoside triphosphate showed different capacities to undergo "replication fork reversal." This reaction occurred at the stalled forks generated by hydroxyurea treatment, was impaired under thermal inactivation of ribonucleoside reductase, and did not take place under thymine starvation.

* Corresponding author. Mailing address: Departamento de Bioquímica, Biología Molecular y Genética, Facultad de Ciencias, Universidad de Extremadura, 06080-Badajoz, Spain. Phone: (34) 924289300, ext. 6955. Fax: (34) 924289687. E-mail: eguzman{at}unex.es

{triangledown} Published ahead of print on 25 May 2007.

Journal of Bacteriology, August 2007, p. 5782-5786, Vol. 189, No. 15
0021-9193/07/$08.00+0     doi:10.1128/JB.00411-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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