This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Alexander, D. C.
Right arrow Articles by Jensen, S. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Alexander, D. C.
Right arrow Articles by Jensen, S. E.

 Previous Article  |  Next Article 

Journal of Bacteriology, August 2007, p. 5867-5874, Vol. 189, No. 16
0021-9193/07/$08.00+0     doi:10.1128/JB.00712-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

pcd Mutants of Streptomyces clavuligerus Still Produce Cephamycin C{triangledown}

Dylan C. Alexander,{dagger} Cecilia L. Anders, Linda Lee,{ddagger} and Susan E. Jensen*

Department of Biological Sciences, University of Alberta, Edmonton, Canada T6G 2E9

Received 4 May 2007/ Accepted 6 June 2007

Biosynthesis of cephamycin C in Streptomyces clavuligerus involves the initial conversion of lysine to {alpha}-aminoadipic acid. Lysine-6-aminotransferase and piperideine-6-carboxylate dehydrogenase carry out this two-step reaction, and genes encoding each of these enzymes are found within the cephamycin C gene cluster. However, while mutation of the lat gene causes complete loss of cephamycin production, pcd mutants still produce cephamycin at 30% to 70% of wild-type levels. Cephamycin production by pcd mutants could be restored to wild-type levels either by supplementation of the growth medium with {alpha}-aminoadipic acid or by complementation of the mutation with an intact copy of the pcd gene. Neither heterologous PCR nor Southern analyses showed any evidence for the presence of a second pcd gene. Furthermore, cell extracts from pcd mutants lack detectable PCD activity. Cephamycin production in the absence of detectable PCD activity suggests that S. clavuligerus must have some alternate means of producing the aminoadipyl-cysteinyl-valine needed for cephamycin biosynthesis.

* Corresponding author. Mailing address: Department of Biological Sciences, University of Alberta, Edmonton, Canada T6G 2E9. Phone: (780) 492-0830. Fax: (780) 492-9234. E-mail: susan.jensen{at}ualberta.ca

{triangledown} Published ahead of print on 15 June 2007.

{dagger} Present address: Cubist Pharmaceuticals, 65 Hayden Avenue, Lexington, MA 02421.

{ddagger} Dept. of Molecular & Cell Biology, University of California, Berkeley, Berkeley, CA 94720-3202.

Journal of Bacteriology, August 2007, p. 5867-5874, Vol. 189, No. 16
0021-9193/07/$08.00+0     doi:10.1128/JB.00712-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»