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A Second Large Plasmid Encodes Conjugative Transfer and Antimicrobial Resistance in O119:H2 and Some Typical O111 Enteropathogenic Escherichia coli Strains ^,

Adaobi I. Nwaneshiudu,^1, Tania Mucci,^1, Derek J. Pickard,² and Iruka N. Okeke^1*

Department of Biology, Haverford College, Pennsylvania,¹ The Wellcome Trust Sanger Institute, Hinxton, Cambridge, United Kingdom²

Received 8 March 2007/ Accepted 6 June 2007

A novel and functional conjugative transfer system identified in O119:H2 enteropathogenic Escherichia coli (EPEC) strain MB80 by subtractive hybridization is encoded on a large multidrug resistance plasmid, distinct from the well-described EPEC adherence factor (EAF) plasmid. Variants of the MB80 conjugative resistance plasmid were identified in other EPEC strains, including the prototypical O111:NM strain B171, from which the EAF plasmid has been sequenced. This separate large plasmid and the selective advantage that it confers in the antibiotic era have been overlooked because it comigrates with the virulence plasmid on conventional gels.

Published ahead of print on 15 June 2007.

Supplemental material for this article may be found at http://jb.asm.org/.

Present address: Department of Microbiology and Immunology, Temple University School of Medicine, 3420 N. Broad St., Philadelphia, PA 19140.

Present address: Thomas Jefferson University School of Medicine, 1020 Walnut Street, Philadelphia, PA 19107.