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Identification of the Major Protein Component of the Pneumococcal Eclipse Complex ^,

Donald A. Morrison,^2,* Isabelle Mortier-Barrière,^1, Laetitia Attaiech,¹ and Jean-Pierre Claverys¹

Laboratoire de Microbiologie et Génétique Moléculaires, UMR 5100 CNRS-Université Paul Sabatier, 118 route de Narbonne, 31062 Toulouse Cedex 9, France,¹ Laboratory for Molecular Biology, Department of Biological Sciences, University of Illinois at Chicago, 900 South Ashland Ave., Chicago, Illinois 60607²

Received 1 May 2007/ Accepted 19 June 2007

During genetic transformation of Streptococcus pneumoniae, single strands from native donor DNA enter competent cells, where they associate with an unidentified protein with a molecular mass of 15 to 20 kDa to form the eclipse complex. Using Western blotting, we identify the principal protein cofractionating with donor DNA in this complex as SsbB.

Published ahead of print on 29 June 2007.

Supplemental material for this article may be found at http://jb.asm.org/.

These authors contributed equally to this work, which was essentially carried out during a visit of D.A.M. in Toulouse.