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Journal of Bacteriology, September 2007, p. 6501-6505, Vol. 189, No. 17
0021-9193/07/$08.00+0     doi:10.1128/JB.00491-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Artificial Control of Nitrate Respiration through the lac Promoter Permits the Assessment of Oxygen-Mediated Posttranslational Regulation of the nar Operon in Pseudomonas aeruginosa{triangledown}

Chris E. Noriega,{dagger} Vandana Sharma,{ddagger} and John J. Rowe*

Department of Biology, University of Dayton, Dayton, Ohio 45469

Received 30 March 2007/ Accepted 22 June 2007

In this study, oxygen and nitrate regulation of transcription and subsequent protein expression of the unique narK1K2GHJI respiratory operon of Pseudomonas aeruginosa were investigated. Under the control of PLAC, P. aeruginosa was able to transcribe nar and subsequently express methyl viologen-linked nitrate reductase activity under aerobic conditions without nitrate. Modulation of PLAC through the LacI repressor enabled us to assess both transcriptional and posttranslational regulation by oxygen during physiological whole-cell nitrate reduction.

* Corresponding author. Mailing address: Department of Biology, University of Dayton, 300 College Park, Dayton, OH 45469-2320. Phone: (937) 229-2521. Fax: (937) 229-2021. E-mail: John.Rowe{at}notes.udayton.edu

{triangledown} Published ahead of print on 6 July 2007.

{dagger} Present address: Section of Microbiology, University of California, Davis, CA 95616-8665.

{ddagger} Present address: Department of Biomedical Engineering, University of California, Davis, CA 95616-5294.

Journal of Bacteriology, September 2007, p. 6501-6505, Vol. 189, No. 17
0021-9193/07/$08.00+0     doi:10.1128/JB.00491-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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