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SpxB Is a Suicide Gene of Streptococcus pneumoniae and Confers a Selective Advantage in an In Vivo Competitive Colonization Model

Gili Regev-Yochay,^1,2* Krzysztof Trzcinski,¹ Claudette M. Thompson,¹ Marc Lipsitch,^1, and Richard Malley^2,

Department of Epidemiology and Department of Immunology and Infectious Diseases, Harvard School of Public Health,¹ Division of Infectious Diseases, Department of Medicine, Children's Hospital, Harvard Medical School, Boston, Massachusetts²

Received 24 May 2007/ Accepted 9 July 2007

The human bacterial pathogen Streptococcus pneumoniae dies spontaneously upon reaching stationary phase. The extent of S. pneumoniae death at stationary phase is unusual in bacteria and has been conventionally attributed to autolysis by the LytA amidase. In this study, we show that spontaneous pneumococcal death is due to hydrogen peroxide (H₂O₂), not LytA, and that the gene responsible for H₂O₂ production (spxB) also confers a survival advantage in colonization. Survival of S. pneumoniae in stationary phase was significantly prolonged by eliminating H₂O₂ in any of three ways: chemically by supplementing the media with catalase, metabolically by growing the bacteria under anaerobic conditions, or genetically by constructing spxB mutants that do not produce H₂O₂. Likewise, addition of H₂O₂ to exponentially growing S. pneumoniae resulted in a death rate similar to that of cells in stationary phase. While lytA mutants did not lyse at stationary phase, they died at a rate similar to that of the wild-type strain. Furthermore, we show that the death process induced by H₂O₂ has features of apoptosis, as evidenced by increased annexin V staining, decreased DNA content, and appearance as assessed by transmission electron microscopy. Finally, in an in vivo rat model of competitive colonization, the presence of spxB conferred a selective advantage over the spxB mutant, suggesting an explanation for the persistence of this gene. We conclude that a suicide gene of pneumococcus is spxB, which induces an apoptosis-like death in pneumococci and confers a selective advantage in nasopharyngeal cocolonization.

Published ahead of print on 13 July 2007.

These two authors contributed equally to this work.

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