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Journal of Bacteriology, September 2007, p. 6602-6610, Vol. 189, No. 18
0021-9193/07/$08.00+0     doi:10.1128/JB.00679-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Simultaneous Catabolite Repression between Glucose and Toluene Metabolism in Pseudomonas putida Is Channeled through Different Signaling Pathways{triangledown} ,{dagger}

Teresa del Castillo and Juan L. Ramos*

Consejo Superior de Investigaciones Científicas, Estación Experimental del Zaidín, Department of Environmental Protection, C/ Prof. Albareda, 1, E-18008 Granada, Spain

Received 30 April 2007/ Accepted 2 July 2007

Pseudomonas putida KT2440(pWW0) can use toluene via the TOL plasmid-encoded catabolic pathways and can use glucose via a series of three peripheral chromosome-encoded routes that convert glucose into 6-phosphogluconate (6PG), namely, the glucokinase pathway, in which glucose is transformed to 6PG through the action of glucokinase and glucose-6-phosphate dehydrogenase. Alternatively, glucose can be oxidized to gluconate, which can be phosphorylated by gluconokinase to 6PG or oxidized to 2-ketogluconate, which, in turn, is converted into 6PG. Our results show that KT2440 metabolizes glucose and toluene simultaneously, as revealed by net flux analysis of [13C]glucose. Determination of glucokinase and gluconokinase activities in glucose metabolism, gene expression assays using a fusion of the promoter of the Pu TOL upper pathway to 'lacZ, and global transcriptomic assays revealed simultaneous catabolite repression in the use of these two carbon sources. The effect of toluene on glucose metabolism was directed to the glucokinase branch and did not affect gluconate metabolism. Catabolite repression of the glucokinase pathway and the TOL pathway was triggered by two different catabolite repression systems. Expression from Pu was repressed mainly via PtsN in response to high levels of 2-dehydro-3-deoxygluconate-6-phosphate, whereas repression of the glucokinase pathway was channeled through Crc.

* Corresponding author. Mailing address: EEZ-CSIC, C/ Prof. Albareda, 1, E-18008 Granada, Spain. Phone: 34 958 181600. Fax: 34 958 135740. E-mail: jlramos{at}eez.csic.es

{triangledown} Published ahead of print on 6 July 2007.

{dagger} Supplemental material for this article may be found at http://jb.asm.org/.

Journal of Bacteriology, September 2007, p. 6602-6610, Vol. 189, No. 18
0021-9193/07/$08.00+0     doi:10.1128/JB.00679-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Kim, J., Yeom, J., Jeon, C. O., Park, W. (2009). Intracellular 2-keto-3-deoxy-6-phosphogluconate is the signal for carbon catabolite repression of phenylacetic acid metabolism in Pseudomonas putida KT2440. Microbiology 155: 2420-2428 [Abstract] [Full Text]  
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  • Pfluger, K., de Lorenzo, V. (2008). Evidence of In Vivo Cross Talk between the Nitrogen-Related and Fructose-Related Branches of the Carbohydrate Phosphotransferase System of Pseudomonas putida. J. Bacteriol. 190: 3374-3380 [Abstract] [Full Text]  
  • del Castillo, T., Duque, E., Ramos, J. L. (2008). A Set of Activators and Repressors Control Peripheral Glucose Pathways in Pseudomonas putida To Yield a Common Central Intermediate. J. Bacteriol. 190: 2331-2339 [Abstract] [Full Text]  


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