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Journal of Bacteriology, September 2007, p. 6723-6726, Vol. 189, No. 18
0021-9193/07/$08.00+0     doi:10.1128/JB.00279-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Characterization of In Vitro Chlamydial Cultures in Low-Oxygen Atmospheres{triangledown} ,{dagger}

Nicolai Juul,1 Helene Jensen,1,2 Malene Hvid,1 Gunna Christiansen,1 and Svend Birkelund1*

Institute of Medical Microbiology and Immunology,1 Institute of Biology, University of Aarhus, Aarhus, Denmark2

Received 20 February 2007/ Accepted 4 July 2007

To mimic in vivo conditions during chlamydial infections, Chlamydia trachomatis serovar D and Chlamydia pneumoniae CWL029 were cultured in low-oxygen atmospheres containing 4% O2, with parallel controls cultured in atmospheric air. Both were enriched with 5% CO2. The results showed a dramatic increase in the growth of C. pneumoniae but not of C. trachomatis.

* Corresponding author. Mailing address: Institute of Medical Microbiology and Immunology, Bartholin Building, University of Aarhus, DK-8000 Aarhus C, Denmark. Phone: 45 89421758. Fax: 45 86196128. E-mail: chlam{at}medmicro.au.dk

{triangledown} Published ahead of print on 13 July 2007.

{dagger} Supplemental material for this article may be found at http://jb.asm.org/.

Journal of Bacteriology, September 2007, p. 6723-6726, Vol. 189, No. 18
0021-9193/07/$08.00+0     doi:10.1128/JB.00279-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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