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Journal of Bacteriology, October 2007, p. 6743-6750, Vol. 189, No. 19
0021-9193/07/$08.00+0     doi:10.1128/JB.00889-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Transcriptomic Analysis of the Sulfate Starvation Response of Pseudomonas aeruginosa{triangledown} ,{dagger}

Tewes Tralau,1 Stéphane Vuilleumier,2 Christelle Thibault,2 Barry J. Campbell,3 C. Anthony Hart,3 and Michael A. Kertesz1*

Faculty of Life Sciences, University of Manchester, Oxford Road, Manchester M13 9PT, United Kingdom,1 Génétique Moléculaire, Génomique, Microbiologie, UMR 7156 Université Louis Pasteur—CNRS, F-67083 Strasbourg, France,2 Faculty of Medicine, University of Liverpool, Liverpool L69 3GA, United Kingdom3

Received 7 June 2007/ Accepted 25 July 2007

Pseudomonas aeruginosa is an opportunistic pathogen that causes a number of infections in humans, but is best known for its association with cystic fibrosis. It is able to use a wide range of sulfur compounds as sources of sulfur for growth. Gene expression in response to changes in sulfur supply was studied in P. aeruginosa E601, a cystic fibrosis isolate that displays mucin sulfatase activity, and in P. aeruginosa PAO1. A large family of genes was found to be upregulated by sulfate limitation in both isolates, encoding sulfatases and sulfonatases, transport systems, oxidative stress proteins, and a sulfate-regulated TonB/ExbBD complex. These genes were localized in five distinct islands on the genome and encoded proteins with a significantly reduced content of cysteine and methionine. Growth of P. aeruginosa E601 with mucin as the sulfur source led not only to a sulfate starvation response but also to induction of genes involved with type III secretion systems.

* Corresponding author. Mailing address: Faculty of Life Sciences, University of Manchester, Michael Smith Bldg., Oxford Rd., Manchester M13 9PT, England. Phone: 44-161-275 3895. Fax: 44-161-275 5082. E-mail: michael.kertesz{at}manchester.ac.uk

{triangledown} Published ahead of print on 3 August 2007.

{dagger} Supplemental material for this article may be found at http://jb.asm.org/.

Journal of Bacteriology, October 2007, p. 6743-6750, Vol. 189, No. 19
0021-9193/07/$08.00+0     doi:10.1128/JB.00889-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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