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Journal of Bacteriology, October 2007, p. 6891-6900, Vol. 189, No. 19
0021-9193/07/$08.00+0     doi:10.1128/JB.00819-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Correlation between Growth Rates, EIIACrr Phosphorylation, and Intracellular Cyclic AMP Levels in Escherichia coli K-12{triangledown}

Katja Bettenbrock,1* Thomas Sauter,2 Knut Jahreis,3 Andreas Kremling,1 Joseph W. Lengeler,1 and Ernst-Dieter Gilles1

MPI für Dynamik Komplexer Technischer Systeme, Sandtorstr. 1, 39106 Magdeburg, Germany,1 Institut für Systemdynamik, Universität Stuttgart, Pfaffenwaldring 9, 70569 Stuttgart, Germany,2 AG Genetik, Universität Osnabrück, 49069 Osnabrück, Germany3

Received 25 May 2007/ Accepted 20 July 2007

In Escherichia coli K-12, components of the phosphoenolpyruvate-dependent phosphotransferase systems (PTSs) represent a signal transduction system involved in the global control of carbon catabolism through inducer exclusion mediated by phosphoenolpyruvate-dependent protein kinase enzyme IIACrr (EIIACrr) (= EIIAGlc) and catabolite repression mediated by the global regulator cyclic AMP (cAMP)-cAMP receptor protein (CRP). We measured in a systematic way the relation between cellular growth rates and the key parameters of catabolite repression, i.e., the phosphorylated EIIACrr (EIIACrr~P) level and the cAMP level, using in vitro and in vivo assays. Different growth rates were obtained by using either various carbon sources or by growing the cells with limited concentrations of glucose, sucrose, and mannitol in continuous bioreactor experiments. The ratio of EIIACrr to EIIACrr~P and the intracellular cAMP concentrations, deduced from the activity of a cAMP-CRP-dependent promoter, correlated well with specific growth rates between 0.3 h–1 and 0.7 h–1, corresponding to generation times of about 138 and 60 min, respectively. Below and above this range, these parameters were increasingly uncoupled from the growth rate, which perhaps indicates an increasing role executed by other global control systems, in particular the stringent-relaxed response system.

* Corresponding author. Mailing address: MPI für Dynamik Komplexer Technischer Systeme, Sandtorstr.1, 39106 Magdeburg, Germany. Phone: 49 391 6110249. Fax: 49 391 6110510. E-mail: bettenbrock{at}mpi-magdeburg.mpg.de

{triangledown} Published ahead of print on 3 August 2007.

Journal of Bacteriology, October 2007, p. 6891-6900, Vol. 189, No. 19
0021-9193/07/$08.00+0     doi:10.1128/JB.00819-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Singh, K. D., Schmalisch, M. H., Stulke, J., Gorke, B. (2008). Carbon Catabolite Repression in Bacillus subtilis: Quantitative Analysis of Repression Exerted by Different Carbon Sources. J. Bacteriol. 190: 7275-7284 [Abstract] [Full Text]  
  • Kremling, A., Bettenbrock, K., Gilles, E. D. (2008). A feed-forward loop guarantees robust behavior in Escherichia coli carbohydrate uptake. Bioinformatics 24: 704-710 [Abstract] [Full Text]  


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