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Journal of Bacteriology, October 2007, p. 7024-7031, Vol. 189, No. 19
0021-9193/07/$08.00+0     doi:10.1128/JB.00710-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Alterations in the Two Globular Domains or in the Connecting {alpha}-Helix of Bacterial Ribosomal Protein L9 Induces +1 Frameshifts{triangledown}

Ramune Leipuviene{dagger} and Glenn R. Björk*

Department of Molecular Biology, Umeå University, S-90187 Umeå, Sweden

Received 3 May 2007/ Accepted 17 July 2007

The ribosomal 50S subunit protein L9, encoded by the gene rplI, is an elongated protein with an {alpha}-helix connecting the N- and C-terminal globular domains. We isolated rplI mutants that suppress the +1 frameshift mutation hisC3072 in Salmonella enterica serovar Typhimurium. These mutants have amino acid substitutions in the N-terminal domain (G24D) or in the C-terminal domain (I94S, A102D, G126V, and F132S) of L9. In addition, different one-base deletions in rplI altered either the final portion of the C terminus or removed the C-terminal domain with or without the connecting {alpha}-helix. An alanine-to-proline substitution at position 59 (A59P), which breaks the {alpha}-helix between the globular domains, induced +1 frameshifting, suggesting that the geometrical relationship between the N and C domains is important to maintain the reading frame. Except for the alterations G126V in the C terminus and A59P in the connecting {alpha}-helix, our results confirm earlier results obtained by using the phage T4 gene 60-based system to monitor bypassing. The way rplI mutations suppress various frameshift mutations suggests that bypassing of many codons from several takeoff and landing sites occurred instead of a specific frameshift forward at overlapping codons.

* Corresponding author. Mailing address: Department of Molecular Biology, Umeå University, S-90187 Umeå, Sweden. Phone: 46-90-7856756. Fax: 46-90-772630. E-mail: glenn.bjork{at}molbiol.umu.se

{triangledown} Published ahead of print on 27 July 2007.

{dagger} Present address: Children's Hospital Oakland Research Institute, 5700 Martin Luther King, Jr. Way, Oakland, CA 94609.

Journal of Bacteriology, October 2007, p. 7024-7031, Vol. 189, No. 19
0021-9193/07/$08.00+0     doi:10.1128/JB.00710-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Atkins, J. F., Bjork, G. R. (2009). A Gripping Tale of Ribosomal Frameshifting: Extragenic Suppressors of Frameshift Mutations Spotlight P-Site Realignment. Microbiol. Mol. Biol. Rev. 73: 178-210 [Abstract] [Full Text]  


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