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Journal of Bacteriology, October 2007, p. 7145-7150, Vol. 189, No. 19
0021-9193/07/$08.00+0     doi:10.1128/JB.00892-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Flavobacterium johnsoniae SprA Is a Cell Surface Protein Involved in Gliding Motility{triangledown}

Shawn S. Nelson, Padden P. Glocka, Sarika Agarwal, David P. Grimm, and Mark J. McBride*

Department of Biological Sciences, University of Wisconsin—Milwaukee, P. O. Box 413, Milwaukee, Wisconsin 53201

Received 7 June 2007/ Accepted 13 July 2007

Flavobacterium johnsoniae cells glide rapidly over surfaces by an unknown mechanism. Transposon-induced sprA mutants formed nonspreading colonies on agar, and the cells examined in wet mounts were deficient in attachment to surfaces and were almost completely nonmotile. Exposure of intact cells to proteinase K cleaved the 270-kDa SprA into several large peptides, suggesting that it is partially exposed on the cell surface.

* Corresponding author. Mailing address: Department of Biological Sciences, 181 Lapham Hall, University of Wisconsin—Milwaukee, 3209 N. Maryland Ave., Milwaukee, WI 53211. Phone: (414) 229-5844. Fax: (414) 229-3926. E-mail: mcbride{at}uwm.edu

{triangledown} Published ahead of print on 20 July 2007.

Journal of Bacteriology, October 2007, p. 7145-7150, Vol. 189, No. 19
0021-9193/07/$08.00+0     doi:10.1128/JB.00892-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • McBride, M. J., Xie, G., Martens, E. C., Lapidus, A., Henrissat, B., Rhodes, R. G., Goltsman, E., Wang, W., Xu, J., Hunnicutt, D. W., Staroscik, A. M., Hoover, T. R., Cheng, Y.-Q., Stein, J. L. (2009). Novel Features of the Polysaccharide-Digesting Gliding Bacterium Flavobacterium johnsoniae as Revealed by Genome Sequence Analysis. Appl. Environ. Microbiol. 75: 6864-6875 [Abstract] [Full Text]  
  • Nelson, S. S., Bollampalli, S., McBride, M. J. (2008). SprB Is a Cell Surface Component of the Flavobacterium johnsoniae Gliding Motility Machinery. J. Bacteriol. 190: 2851-2857 [Abstract] [Full Text]  


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