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High-Affinity Interaction between the S-Layer Protein SbsC and the Secondary Cell Wall Polymer of Geobacillus stearothermophilus ATCC 12980 Determined by Surface Plasmon Resonance Technology ^,

Center for NanoBiotechnology, University of Natural Resources and Applied Life Sciences, A-1180 Vienna,¹ Bio-Products and Bio-Engineering Aktiengesellschaft/Biomedizinische Forschungsgesellschaft mbH, A-1090 Vienna, Austria²

Received 27 February 2007/ Accepted 16 July 2007

Surface plasmon resonance studies using C-terminal truncation forms of the S-layer protein SbsC (recombinant SbsC consisting of amino acids 31 to 270 [rSbsC_31-270] and rSbsC_31-443) and the secondary cell wall polymer (SCWP) isolated from Geobacillus stearothermophilus ATCC 12980 confirmed the exclusive responsibility of the N-terminal region comprising amino acids 31 to 270 for SCWP binding. Quantitative analyses indicated binding behavior demonstrating low, medium, and high affinities.

Published ahead of print on 20 July 2007.

Dedicated to the memory of Margit Sára.