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Journal of Bacteriology, January 2007, p. 342-350, Vol. 189, No. 2
0021-9193/07/$08.00+0     doi:10.1128/JB.01472-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Molecular Evolution of Typical Enteropathogenic Escherichia coli: Clonal Analysis by Multilocus Sequence Typing and Virulence Gene Allelic Profiling{triangledown} ,{dagger}

David W. Lacher,1 Hans Steinsland,1,2 T. Eric Blank,3 Michael S. Donnenberg,3 and Thomas S. Whittam1*

Microbial Evolution Laboratory, National Food Safety & Toxicology Center, Michigan State University, East Lansing, Michigan,1 Centre for International Health, University of Bergen, Bergen, Norway,2 Division of Infectious Diseases, Department of Medicine, University of Maryland School of Medicine, Baltimore, Maryland 212013

Received 18 September 2006/ Accepted 30 October 2006

Enteropathogenic Escherichia coli (EPEC) infections are a leading cause of infantile diarrhea in developing nations. Typical EPEC isolates are differentiated from other types of pathogenic E. coli by two distinctive phenotypes, attaching effacement and localized adherence. The genes specifying these phenotypes are found on the locus of enterocyte effacement (LEE) and the EPEC adherence factor (EAF) plasmid. To describe how typical EPEC has evolved, we characterized a diverse collection of strains by multilocus sequence typing (MLST) and performed restriction fragment length polymorphism (RFLP) analysis of three virulence genes (eae, bfpA, and perA) to assess allelic variation. Among 129 strains representing 20 O-serogroups, 21 clonal genotypes were identified using MLST. RFLP analysis resolved nine eae, nine bfpA, and four perA alleles. Each bfpA allele was associated with only one perA allele class, suggesting that recombination has not played a large role in shuffling the bfpA and perA loci between separate EAF plasmids. The distribution of eae alleles among typical EPEC strains is more concordant with the clonal relationships than the distribution of the EAF plasmid types. These results provide further support for the hypothesis that the EPEC pathotype has evolved multiple times within E. coli through separate acquisitions of the LEE island and EAF plasmid.

* Corresponding author. Mailing address: National Food Safety & Toxicology Center, 165 Food Safety & Toxicology Building, Michigan State University, East Lansing, MI 48824. Phone: (517) 432-3100, ext. 178. Fax: (517) 432-2310. E-mail: whittam{at}msu.edu.

{triangledown} Published ahead of print on 10 November 2006.

{dagger} Supplemental material for this article may be found at http://jb.asm.org/.

Journal of Bacteriology, January 2007, p. 342-350, Vol. 189, No. 2
0021-9193/07/$08.00+0     doi:10.1128/JB.01472-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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