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Journal of Bacteriology, January 2007, p. 403-409, Vol. 189, No. 2
0021-9193/07/$08.00+0     doi:10.1128/JB.01246-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

CC1, a Novel Crenarchaeal DNA Binding Protein{triangledown}

Xiao Luo,1 Uli Schwarz-Linek,1 Catherine H. Botting,1 Reinhard Hensel,2 Bettina Siebers,2 and Malcolm F. White1*

Centre for Biomolecular Sciences, University of St. Andrews, St. Andrews, Fife KY16 9ST, United Kingdom,1 Microbiology I, University Duisburg-Essen, Universitätsstr. 5, 45117 Essen, Germany2

Received 8 August 2006/ Accepted 21 October 2006

The genomes of the related crenarchaea Pyrobaculum aerophilum and Thermoproteus tenax lack any obvious gene encoding a single-stranded DNA binding protein (SSB). SSBs are essential for DNA replication, recombination, and repair and are found in all other genomes across the three domains of life. These two archaeal genomes also have only one identifiable gene encoding a chromatin protein (the Alba protein), while most other archaea have at least two different abundant chromatin proteins. We performed a biochemical screen for novel nucleic acid binding proteins present in cell extracts of T. tenax. An assay for proteins capable of binding to a single-stranded DNA oligonucleotide resulted in identification of three proteins. The first protein, Alba, has been shown previously to bind single-stranded DNA as well as duplex DNA. The two other proteins, which we designated CC1 (for crenarchaeal chromatin protein 1), are very closely related to one another, and homologs are restricted to the P. aerophilum and Aeropyrum pernix genomes. CC1 is a 6-kDa, monomeric, basic protein that is expressed at a high level in T. tenax. This protein binds single- and double-stranded DNAs with similar affinities. These properties are consistent with a role for CC1 as a crenarchaeal chromatin protein.

* Corresponding author. Mailing address: Centre for Biomolecular Sciences, University of St. Andrews, St. Andrews, Fife KY16 9ST, United Kingdom. Phone: 44-1334-463432. Fax: 44-1334-462595. E-mail: mfw2{at}st-and.ac.uk.

{triangledown} Published ahead of print on 3 November 2006.

Journal of Bacteriology, January 2007, p. 403-409, Vol. 189, No. 2
0021-9193/07/$08.00+0     doi:10.1128/JB.01246-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Haseltine, C. A., Kowalczykowski, S. C. (2009). An archaeal Rad54 protein remodels DNA and stimulates DNA strand exchange by RadA. Nucleic Acids Res 37: 2757-2770 [Abstract] [Full Text]  
  • Guo, L., Feng, Y., Zhang, Z., Yao, H., Luo, Y., Wang, J., Huang, L. (2008). Biochemical and structural characterization of Cren7, a novel chromatin protein conserved among Crenarchaea. Nucleic Acids Res 36: 1129-1137 [Abstract] [Full Text]  


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