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Journal of Bacteriology, January 2007, p. 540-550, Vol. 189, No. 2
0021-9193/07/$08.00+0     doi:10.1128/JB.01260-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Role of Gne and GalE in the Virulence of Aeromonas hydrophila Serotype O34{triangledown}

Rocío Canals, Natalia Jiménez, Silvia Vilches, Miguel Regué, Susana Merino, and Juan M. Tomás*

Departamento de Microbiología, Facultad de Biología, Universidad de Barcelona, Diagonal 645, 08071 Barcelona, Spain

Received 10 August 2006/ Accepted 31 October 2006

The mesophilic Aeromonas hydrophila AH-3 (serotype O34) strain shows two different UDP-hexose epimerases in its genome: GalE (EC 3.1.5.2) and Gne (EC 3.1.5.7). Similar homologues were detected in the different mesophilic Aeromonas strains tested. GalE shows only UDP-galactose 4-epimerase activity, while Gne is able to perform a dual activity (mainly UDP-N-acetyl galactosamine 4-epimerase and also UDP-galactose 4-epimerase). We studied the activities in vitro of both epimerases and also in vivo through the lipopolysaccharide (LPS) structure of A. hydrophila gne mutants, A. hydrophila galE mutants, A. hydrophila galE-gne double mutants, and independently complemented mutants with both genes. Furthermore, the enzymatic activity in vivo, which renders different LPS structures on the mentioned A. hydrophila mutant strains or the complemented mutants, allowed us to confirm a clear relationship between the virulence of these strains and the presence/absence of the O34 antigen LPS.

* Corresponding author. Mailing address: Departamento Microbiología, Facultad Biología, Universidad Barcelona, Diagonal 645, 08071 Barcelona, Spain. Phone: 34-93-4021486. Fax: 34-93-4039047. E-mail: jtomas{at}ub.edu.

{triangledown} Published ahead of print on 10 November 2006.

Journal of Bacteriology, January 2007, p. 540-550, Vol. 189, No. 2
0021-9193/07/$08.00+0     doi:10.1128/JB.01260-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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