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Journal of Bacteriology, January 2007, p. 656-662, Vol. 189, No. 2
0021-9193/07/$08.00+0     doi:10.1128/JB.01194-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Respiratory Nitrate Ammonification by Shewanella oneidensis MR-1{triangledown}

Claribel Cruz-García,1,2,{dagger} Alison E. Murray,1,{ddagger} Joel A. Klappenbach,1,§ Valley Stewart,3 and James M. Tiedje1,2,4*

Center for Microbial Ecology,1 Department of Crop & Soil Sciences,2 Department of Microbiology & Molecular Genetics, Michigan State University, East Lansing, Michigan 48824-1325,4 Section of Microbiology, University of California, Davis, California 95616-86653

Received 1 August 2006/ Accepted 27 October 2006

Anaerobic cultures of Shewanella oneidensis MR-1 grown with nitrate as the sole electron acceptor exhibited sequential reduction of nitrate to nitrite and then to ammonium. Little dinitrogen and nitrous oxide were detected, and no growth occurred on nitrous oxide. A mutant with the napA gene encoding periplasmic nitrate reductase deleted could not respire or assimilate nitrate and did not express nitrate reductase activity, confirming that the NapA enzyme is the sole nitrate reductase. Hence, S. oneidensis MR-1 conducts respiratory nitrate ammonification, also termed dissimilatory nitrate reduction to ammonium, but not respiratory denitrification.

* Corresponding author. Mailing address: Center for Microbial Ecology, 540 Plant and Soil Sciences Building, Michigan State University, East Lansing, MI 48824-1325. Phone: (517) 353-9021. Fax: (517) 353-2917. E-mail: tiedjej{at}msu.edu.

{triangledown} Published ahead of print on 10 November 2006.

{dagger} Present address: School of Civil and Environmental Engineering, Georgia Institute of Technology, Atlanta, GA 30332.

{ddagger} Present address: Division of Earth & Ecosystem Sciences, Desert Research Institute, Reno, NV 89512.

§ Present address: Rosetta Inpharmatics/Merck Research Laboratories, Seattle, WA 98109.

Journal of Bacteriology, January 2007, p. 656-662, Vol. 189, No. 2
0021-9193/07/$08.00+0     doi:10.1128/JB.01194-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Lin, H.-Y., Bledsoe, P. J., Stewart, V. (2007). Activation of yeaR-yoaG Operon Transcription by the Nitrate-Responsive Regulator NarL Is Independent of Oxygen- Responsive Regulator Fnr in Escherichia coli K-12. J. Bacteriol. 189: 7539-7548 [Abstract] [Full Text]  


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