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Journal of Bacteriology, October 2007, p. 7367-7375, Vol. 189, No. 20
0021-9193/07/$08.00+0     doi:10.1128/JB.00590-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Characterization of Riboflavin (Vitamin B2) Transport Proteins from Bacillus subtilis and Corynebacterium glutamicum{triangledown}

Christian Vogl,1 Simon Grill,2 Oliver Schilling,3 Jörg Stülke,3 Matthias Mack,2 and Jürgen Stolz1*

Lehrstuhl für Zellbiologie und Pflanzenphysiologie, Universität Regensburg, Universitätsstrasse 31, 93040 Regensburg, Germany,1 Institut für Technische Mikrobiologie, Hochschule Mannheim, Windeckstrasse 110, 68163 Mannheim, Germany,2 Institut für Mikrobiologie und Genetik, Abteilung für Allgemeine Mikrobiologie, Grisebachstr. 8, 37077 Göttingen, Germany3

Received 17 April 2007/ Accepted 3 August 2007

Riboflavin (vitamin B2) is the direct precursor of the flavin cofactors flavin mononucleotide and flavin adenine dinucleotide, essential components of cellular biochemistry. In this work we investigated the unrelated proteins YpaA from Bacillus subtilis and PnuX from Corynebacterium glutamicum for a role in riboflavin uptake. Based on the regulation of the corresponding genes by a riboswitch mechanism, both proteins have been predicted to be involved in flavin metabolism. Moreover, their primary structures suggested that these proteins integrate into the cytoplasmic membrane. We provide experimental evidence that YpaA is a plasma membrane protein with five transmembrane domains and a cytoplasmic C terminus. In B. subtilis, riboflavin uptake was increased when ypaA was overexpressed and abolished when ypaA was deleted. Riboflavin uptake activity and the abundance of the YpaA protein were also increased when riboflavin auxotrophic mutants were grown in limiting amounts of riboflavin. YpaA-mediated riboflavin uptake was sensitive to protonophors and reduced in the absence of glucose, demonstrating that the protein requires metabolic energy for substrate translocation. In addition, we demonstrate that PnuX from C. glutamicum also is a riboflavin transporter. Transport by PnuX was not energy dependent and had high apparent affinity for riboflavin (Km 11 µM). Roseoflavin, a toxic riboflavin analog, appears to be a substrate of PnuX and YpaA. We propose to designate the gene names ribU for ypaA and ribM for pnuX to reflect that the encoded proteins function in riboflavin uptake and that the genes have different phylogenetic origins.

* Corresponding author. Present address: Lehrstuhl für Ernährungsphysiologie, Technische Universität München, Am Forum 5, 85350 Freising-Weihenstephan, Germany. Phone: 49 8161 71 2359. Fax: 49 8161 71 3999. E-mail: juergen.stolz{at}wzw.tum.de

{triangledown} Published ahead of print on 10 August 2007.

Journal of Bacteriology, October 2007, p. 7367-7375, Vol. 189, No. 20
0021-9193/07/$08.00+0     doi:10.1128/JB.00590-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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