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Journal of Bacteriology, October 2007, p. 7503-7506, Vol. 189, No. 20
0021-9193/07/$08.00+0     doi:10.1128/JB.00957-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Cell Surface Filaments of the Gliding Bacterium Flavobacterium johnsoniae Revealed by Cryo-Electron Tomography{triangledown} ,{dagger}

Jun Liu,1 Mark J. McBride,2* and Sriram Subramaniam1*

Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892,1 Department of Biological Sciences, University of Wisconsin—Milwaukee, Milwaukee, Wisconsin 532012

Received 17 June 2007/ Accepted 2 August 2007

Flavobacterium johnsoniae cells glide rapidly over surfaces by an as-yet-unknown mechanism. Using cryo-electron tomography, we show that wild-type cells display tufts of ~5-nm-wide cell surface filaments that appear to be anchored to the inner surface of the outer membrane. These filaments are absent in cells of a nonmotile gldF mutant but are restored upon expression of plasmid-encoded GldF, a component of a putative ATP-binding cassette transporter.

* Corresponding author. Mailing address for S. Subramaniam: Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892. Phone: (301) 594-2062. Fax: (301) 480-3834. E-mail: ss1{at}nih.gov. Mailing address for M. J. McBride: Department of Biological Sciences, University of Wisconsin—Milwaukee, Milwaukee, WI 53201. Phone: (414) 229-5844. Fax: (414) 229-3926. E-mail: mcbride{at}uwm.edu

{triangledown} Published ahead of print on 10 August 2007.

{dagger} Supplemental material for this article may be found at http://jb.asm.org/.

Journal of Bacteriology, October 2007, p. 7503-7506, Vol. 189, No. 20
0021-9193/07/$08.00+0     doi:10.1128/JB.00957-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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