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Journal of Bacteriology, October 2007, p. 7507-7510, Vol. 189, No. 20
0021-9193/07/$08.00+0     doi:10.1128/JB.00934-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Mutations in Type I and Type IV Pilus Biosynthetic Genes Affect Twitching Motility Rates in Xylella fastidiosa{triangledown} ,{dagger}

Leonardo De La Fuente, Thomas J. Burr, and Harvey C. Hoch*

Department of Plant Pathology, Cornell University—New York State Agricultural Experiment Station, Geneva, New York

Received 13 June 2007/ Accepted 26 July 2007

Xylella fastidiosa possesses both type I and type IV pili at the same cell pole. By use of a microfluidic device, the speed of twitching movement by wild-type cells on a glass surface against the flow direction of media was measured as 0.86 (standard error [SE], 0.04) µm min–1. A type I pilus mutant (fimA) moved six times faster (4.85 [SE, 0.27] µm min–1) and a pilY1 mutant moved three times slower (0.28 [SE, 0.03] µm min–1) than wild-type cells. Type I pili slow the rate of movement, while the putative type IV pilus protein PilY1 is likely important for attachment to surfaces.

* Corresponding author. Mailing address: Department of Plant Pathology, Cornell University—New York State Agricultural Experiment Station, Geneva, NY 14456. Phone: (315) 787-2332. Fax: (315) 787-2389. E-mail: hch1{at}cornell.edu

{triangledown} Published ahead of print on 10 August 2007.

{dagger} Supplemental material for this article may be found at http://jb.asm.org/.

Journal of Bacteriology, October 2007, p. 7507-7510, Vol. 189, No. 20
0021-9193/07/$08.00+0     doi:10.1128/JB.00934-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • De La Fuente, L., Burr, T. J., Hoch, H. C. (2008). Autoaggregation of Xylella fastidiosa Cells Is Influenced by Type I and Type IV Pili. Appl. Environ. Microbiol. 74: 5579-5582 [Abstract] [Full Text]  


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