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Journal of Bacteriology, October 2007, p. 7511-7514, Vol. 189, No. 20
0021-9193/07/$08.00+0     doi:10.1128/JB.00968-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Complex Formation by the mrpABCDEFG Gene Products, Which Constitute a Principal Na+/H+ Antiporter in Bacillus subtilis{triangledown}

Yusuke Kajiyama,1,2 Masato Otagiri,1 Junichi Sekiguchi,2 Saori Kosono,1* and Toshiaki Kudo1,3

Environmental Molecular Biology Laboratory, Wako, Saitama,1 Department of Applied Biology, Shinshu University, Ueda,2 Graduate School of Integrated Science, Yokohama City University, Yokohama, Japan3

Received 19 June 2007/ Accepted 3 August 2007

The Bacillus subtilis Mrp (also referred to as Sha) is a particularly unusual Na+/H+ antiporter encoded by mrpABCDEFG. Using His tagging of Mrp proteins, we showed complex formation by the mrpABCDEFG gene products by pull-down and blue native polyacrylamide gel electrophoresis analyses. This is the first molecular evidence that the Mrp is a multicomponent antiporter in the cation-proton antiporter 3 family.

* Corresponding author. Mailing address: Environmental Molecular Biology Lab., RIKEN, Wako, Saitama 351-0198, Japan. Phone: 81-48-467-9545. Fax: 81-48-462-4672. E-mail: kosono{at}riken.jp

{triangledown} Published ahead of print on 10 August 2007.

Journal of Bacteriology, October 2007, p. 7511-7514, Vol. 189, No. 20
0021-9193/07/$08.00+0     doi:10.1128/JB.00968-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Kajiyama, Y., Otagiri, M., Sekiguchi, J., Kudo, T., Kosono, S. (2009). The MrpA, MrpB and MrpD subunits of the Mrp antiporter complex in Bacillus subtilis contain membrane-embedded and essential acidic residues. Microbiology 155: 2137-2147 [Abstract] [Full Text]  
  • Morino, M., Natsui, S., Swartz, T. H., Krulwich, T. A., Ito, M. (2008). Single Gene Deletions of mrpA to mrpG and mrpE Point Mutations Affect Activity of the Mrp Na+/H+ Antiporter of Alkaliphilic Bacillus and Formation of Hetero-Oligomeric Mrp Complexes. J. Bacteriol. 190: 4162-4172 [Abstract] [Full Text]  


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