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Transducing Particles of Staphylococcus aureus Pathogenicity Island SaPI1 Are Comprised of Helper Phage-Encoded Proteins ^,

Sandra M. Tallent,^1, Timothy B. Langston,² Richard G. Moran,² and Gail E. Christie^1*

Departments of Microbiology and Immunology,¹ Pharmacology and Toxicology, Virginia Commonwealth University School of Medicine, Richmond, Virginia²

Received 10 May 2007/ Accepted 3 August 2007

The relationship between the composition of SaPI1 transducing particles and those of helper phage 80 was investigated by direct comparison of virion proteins. Twelve virion proteins were identified by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and mass spectrometry; all were present in both 80 and SaPI1 virions, and all were encoded by 80. No SaPI1-encoded proteins were detected. This confirms the prediction that SaPI1 is encapsidated in a virion assembled from helper phage-encoded proteins.

Published ahead of print on 10 August 2007.

Supplemental material for this article may be found at http://jb.asm.org/.

Present address: Commonwealth of Virginia, Division of Consolidated Laboratory Services, 600 North 5th Street, Richmond, VA 23219.

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