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Journal of Bacteriology, November 2007, p. 7829-7840, Vol. 189, No. 21
0021-9193/07/$08.00+0     doi:10.1128/JB.01036-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Role of Sigma Factors in Controlling Global Gene Expression in Light/Dark Transitions in the Cyanobacterium Synechocystis sp. Strain PCC 6803{triangledown} ,{dagger}

Tina C. Summerfield and Louis A. Sherman*

Department of Biological Sciences, Purdue University, 201 S. University St., Hansen Hall, West Lafayette, Indiana 47907

Received 29 June 2007/ Accepted 17 August 2007

We report on differential gene expression in the cyanobacterium Synechocystis sp. strain PCC 6803 after light-dark transitions in wild-type, {Delta}sigB, and {Delta}sigD strains. We also studied the effect of day length in the presence of glucose on a {Delta}sigB {Delta}sigE mutant. Our results indicated that the absence of SigB or SigD predominately altered gene expression in the dark or in the light, respectively. In the light, approximately 350 genes displayed transcript levels in the {Delta}sigD strain that were different from those of the wild type, with over 200 of these up-regulated in the mutant. In the dark, removal of SigB altered more than 150 genes, and the levels of 136 of these were increased in the mutant compared to those in the wild type. The removal of both SigB and SigE had a major impact on gene expression under mixotrophic growth conditions and resulted in the inability of cells to grow in the presence of glucose with 8-h light and 16-h dark cycles. Our results indicated the importance of group II {sigma} factors in the global regulation of transcription in this organism and are best explained by using the {sigma} cycle paradigm with the stochastic release model described previously (R. A. Mooney, S. A. Darst, and R. Landick, Mol. Cell 20:335-345, 2005). We combined our results with the total protein levels of the {sigma} factors in the light and dark as calculated previously (S. Imamura, S. Yoshihara, S. Nakano, N. Shiozaki, A. Yamada, K. Tanaka, H. Takahashi, M. Asayama, and M. Shirai, J. Mol. Biol. 325:857-872, 2003; S. Imamura, M. Asayama, H. Takahashi, K. Tanaka, H. Takahashi, and M. Shirai, FEBS Lett. 554:357-362, 2003). Thus, we concluded that the control of global transcription is based on the amount of the various {sigma} factors present and able to bind RNA polymerase.

* Corresponding author. Mailing address: Department of Biological Sciences, Purdue University, 201 S. University St., Hansen Hall, West Lafayette, IN 47907. Phone: (765) 494-8106. Fax: (765) 496-1496. E-mail: lsherman{at}purdue.edu

{triangledown} Published ahead of print on 24 August 2007.

{dagger} Supplemental material for this article may be found at http://jb.asm.org/.

Journal of Bacteriology, November 2007, p. 7829-7840, Vol. 189, No. 21
0021-9193/07/$08.00+0     doi:10.1128/JB.01036-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Pollari, M., Ruotsalainen, V., Rantamaki, S., Tyystjarvi, E., Tyystjarvi, T. (2009). Simultaneous Inactivation of Sigma Factors B and D Interferes with Light Acclimation of the Cyanobacterium Synechocystis sp. Strain PCC 6803. J. Bacteriol. 191: 3992-4001 [Abstract] [Full Text]  
  • Summerfield, T. C., Sherman, L. A. (2008). Global Transcriptional Response of the Alkali-Tolerant Cyanobacterium Synechocystis sp. Strain PCC 6803 to a pH 10 Environment. Appl. Environ. Microbiol. 74: 5276-5284 [Abstract] [Full Text]  
  • Asayama, M., Imamura, S. (2008). Stringent promoter recognition and autoregulation by the group 3 {sigma}-factor SigF in the cyanobacterium Synechocystis sp. strain PCC 6803. Nucleic Acids Res 36: 5297-5305 [Abstract] [Full Text]  
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