This Article Full Text Full Text (PDF) Supplemental material Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Madsen, M. L. Articles by Minion, F. C. Search for Related Content PubMed PubMed Citation Articles by Madsen, M. L. Articles by Minion, F. C.

 Previous Article  |  Next Article 

Journal of Bacteriology, November 2007, p. 7977-7982, Vol. 189, No. 22
0021-9193/07/$08.00+0     doi:10.1128/JB.01068-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Array-Based Genomic Comparative Hybridization Analysis of Field Strains of Mycoplasma hyopneumoniae ^,

Melissa L. Madsen,¹ Michael J. Oneal,¹ Stuart W. Gardner,² Erin L. Strait,¹ Dan Nettleton,² Eileen L. Thacker,¹ and F. Chris Minion^1*

Departments of Veterinary Microbiology and Preventive Medicine,¹ Statistics, Iowa State University, Ames, Iowa²

Received 6 July 2007/ Accepted 4 September 2007

Mycoplasma hyopneumoniae is the causative agent of porcine enzootic pneumonia and a major factor in the porcine respiratory disease complex. A clear understanding of the mechanisms of pathogenesis does not exist, although it is clear that M. hyopneumoniae adheres to porcine ciliated epithelium by action of a protein called P97. Previous studies have shown variation in the gene encoding the P97 cilium adhesin in different strains of M. hyopneumoniae, but the extent of genetic variation among field strains across the genome is not known. Since M. hyopneumoniae is a worldwide problem, it is reasonable to expect that a wide range of genetic variability may exist given all of the different breeds and housing conditions. This variation may impact the overall virulence of a single strain. Using microarray technology, this study examined the potential variation of 14 field strains compared to strain 232, on which the array was based. Genomic DNA was obtained, amplified with TempliPhi, and labeled indirectly with Alexa dyes. After genomic hybridization, the arrays were scanned and data were analyzed using a linear statistical model. The results indicated that genetic variation could be detected in all 14 field strains but across different loci, suggesting that variation occurs throughout the genome. Fifty-nine percent of the variable loci were hypothetical genes. Twenty-two percent of the lipoprotein genes showed variation in at least one field strain. A permutation test identified a location in the M. hyopneumoniae genome where there is spatial clustering of variability between the field strains and strain 232.

---

* Corresponding author. Mailing address: Department of Veterinary Microbiology and Preventive Medicine, Iowa State University, Ames, IA 50011. Phone: (515) 294-6347. Fax: (515) 294-1401. E-mail: fcminion{at}iastate.edu

Published ahead of print on 14 September 2007.

Supplemental material for this article may be found at http://jb.asm.org/.

---

Journal of Bacteriology, November 2007, p. 7977-7982, Vol. 189, No. 22
0021-9193/07/$08.00+0     doi:10.1128/JB.01068-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

This article has been cited by other articles:

• Strait, E. L., Madsen, M. L., Minion, F. C., Christopher-Hennings, J., Dammen, M., Jones, K. R., Thacker, E. L. (2008). Real-Time PCR Assays To Address Genetic Diversity among Strains of Mycoplasma hyopneumoniae. J. Clin. Microbiol. 46: 2491-2498 [Abstract] [Full Text]