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Journal of Bacteriology, November 2007, p. 8088-8098, Vol. 189, No. 22
0021-9193/07/$08.00+0     doi:10.1128/JB.01318-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Functional Characterization of Bacterial Oligosaccharyltransferases Involved in O-Linked Protein Glycosylation{triangledown}

Amirreza Faridmoayer,1,2 Messele A. Fentabil,1,3 Dominic C. Mills,1,2 John S. Klassen,1,3 and Mario F. Feldman1,2*

Alberta Ingenuity Centre for Carbohydrate Science,1 Department of Biological Sciences,2 Department of Chemistry, University of Alberta, Edmonton, Alberta T6G 2E9, Canada3

Received 14 August 2007/ Accepted 10 September 2007

Protein glycosylation is an important posttranslational modification that occurs in all domains of life. Pilins, the structural components of type IV pili, are O glycosylated in Neisseria meningitidis, Neisseria gonorrhoeae, and some strains of Pseudomonas aeruginosa. In this work, we characterized the P. aeruginosa 1244 and N. meningitidis MC58 O glycosylation systems in Escherichia coli. In both cases, sugars are transferred en bloc by an oligosaccharyltransferase (OTase) named PglL in N. meningitidis and PilO in P. aeruginosa. We show that, like PilO, PglL has relaxed glycan specificity. Both OTases are sufficient for glycosylation, but they require translocation of the undecaprenol-pyrophosphate-linked oligosaccharide substrates into the periplasm for activity. Whereas PilO activity is restricted to short oligosaccharides, PglL is able to transfer diverse oligo- and polysaccharides. This functional characterization supports the concept that despite their low sequence similarity, PilO and PglL belong to a new family of "O-OTases" that transfer oligosaccharides from lipid carriers to hydroxylated amino acids in proteins. To date, such activity has not been identified for eukaryotes. To our knowledge, this is the first report describing recombinant O glycoproteins synthesized in E. coli.

* Corresponding author. Mailing address: Department of Biological Sciences, CW405 Biological Sciences Building, University of Alberta, Edmonton, AB T6G 2E9, Canada. Phone: (780) 492-6105. Fax: (780) 492-9234. E-mail: mfeldman{at}ualberta.ca

{triangledown} Published ahead of print on 21 September 2007.

Journal of Bacteriology, November 2007, p. 8088-8098, Vol. 189, No. 22
0021-9193/07/$08.00+0     doi:10.1128/JB.01318-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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