This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Morohoshi, T.
Right arrow Articles by Ikeda, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Morohoshi, T.
Right arrow Articles by Ikeda, T.

 Previous Article  |  Next Article 

Journal of Bacteriology, November 2007, p. 8333-8338, Vol. 189, No. 22
0021-9193/07/$08.00+0     doi:10.1128/JB.01054-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

The Plant Pathogen Pantoea ananatis Produces N-Acylhomoserine Lactone and Causes Center Rot Disease of Onion by Quorum Sensing{triangledown}

Tomohiro Morohoshi,1* Yuta Nakamura,1 Go Yamazaki,2 Akio Ishida,2 Norihiro Kato,1 and Tsukasa Ikeda1

Department of Applied Chemistry, Utsunomiya University, Utsunomiya, Tochigi 321-8585, Japan,1 Department of Environmental Science, Kumamoto University, Kumamoto 860-8555, Japan2

Received 4 July 2007/ Accepted 29 August 2007

A number of gram-negative bacteria have a quorum-sensing system and produce N-acyl-L-homoserine lactone (AHL) that they use them as a quorum-sensing signal molecule. Pantoea ananatis is reported as a common colonist of wheat heads at ripening and causes center rot of onion. In this study, we demonstrated that P. ananatis SK-1 produced two AHLs, N-hexanoyl-L-homoserine lactone (C6-HSL) and N-(3-oxohexanoyl)-L-homoserine lactone (3-oxo-C6-HSL). We cloned the AHL-synthase gene (eanI) and AHL-receptor gene (eanR) and revealed that the deduced amino acid sequence of EanI/EanR showed high identity to those of EsaI/EsaR from P. stewartii. EanR repressed the ean box sequence and the addition of AHLs resulted in derepression of ean box. Inactivation of the chromosomal eanI gene in SK-1 caused disruption of exopolysaccharide (EPS) biosynthesis, biofilm formation, and infection of onion leaves, which were recovered by adding exogenous 3-oxo-C6-HSL. These results demonstrated that the quorum-sensing system involved the biosynthesis of EPS, biofilm formation, and infection of onion leaves in P. ananatis SK-1.

* Corresponding author. Mailing address: Department of Applied Chemistry, Utsunomiya University, 7-1-2 Yoto, Utsunomiya, 321-8585, Japan. Phone and fax: 81-28-689-6176. E-mail: morohosi{at}cc.utsunomiya-u.ac.jp

{triangledown} Published ahead of print on 7 September 2007.

Journal of Bacteriology, November 2007, p. 8333-8338, Vol. 189, No. 22
0021-9193/07/$08.00+0     doi:10.1128/JB.01054-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»