Journal of Bacteriology, November 2007, p. 8353-8356, Vol. 189, No. 22
0021-9193/07/$08.00+0 doi:10.1128/JB.00620-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.
Pseudomonas aeruginosa Psl Is a Galactose- and Mannose-Rich Exopolysaccharide
,
Luyan Ma,1
Haiping Lu,1
April Sprinkle,1
Matthew R. Parsek,2 and
Daniel J. Wozniak1*
Department of Microbiology and Immunology, Wake Forest University School of Medicine, Winston-Salem, North Carolina,1
Department of Microbiology, University of Washington, Seattle, Washington2
Received 21 April 2007/
Accepted 4 July 2007
The Pseudomonas aeruginosa polysaccharide synthesis locus (psl) is predicted to encode an exopolysaccharide which is critical for biofilm formation. Here we used chemical composition analyses and mannose- or galactose-specific lectin staining, followed by confocal laser scanning microscopy and electron microscopy, to show that Psl is a galactose-rich and mannose-rich exopolysaccharide.
* Corresponding author. Mailing address: Department of Microbiology and Immunology, Wake Forest University School of Medicine, Medical Center Blvd., Winston-Salem, NC 27157-1064. Phone: (336) 716-2016. Fax: (336) 716-9928. E-mail: dwozniak{at}wfubmc.edu
Published ahead of print on 13 July 2007.
Supplemental material for this article may be found at http://jb.asm.org/.
Journal of Bacteriology, November 2007, p. 8353-8356, Vol. 189, No. 22
0021-9193/07/$08.00+0 doi:10.1128/JB.00620-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.
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Copyright © 2007 by the American Society for Microbiology. All rights reserved.