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Journal of Bacteriology, November 2007, p. 8353-8356, Vol. 189, No. 22
0021-9193/07/$08.00+0     doi:10.1128/JB.00620-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Pseudomonas aeruginosa Psl Is a Galactose- and Mannose-Rich Exopolysaccharide{triangledown} ,{dagger}

Luyan Ma,1 Haiping Lu,1 April Sprinkle,1 Matthew R. Parsek,2 and Daniel J. Wozniak1*

Department of Microbiology and Immunology, Wake Forest University School of Medicine, Winston-Salem, North Carolina,1 Department of Microbiology, University of Washington, Seattle, Washington2

Received 21 April 2007/ Accepted 4 July 2007

The Pseudomonas aeruginosa polysaccharide synthesis locus (psl) is predicted to encode an exopolysaccharide which is critical for biofilm formation. Here we used chemical composition analyses and mannose- or galactose-specific lectin staining, followed by confocal laser scanning microscopy and electron microscopy, to show that Psl is a galactose-rich and mannose-rich exopolysaccharide.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, Wake Forest University School of Medicine, Medical Center Blvd., Winston-Salem, NC 27157-1064. Phone: (336) 716-2016. Fax: (336) 716-9928. E-mail: dwozniak{at}wfubmc.edu

{triangledown} Published ahead of print on 13 July 2007.

{dagger} Supplemental material for this article may be found at http://jb.asm.org/.

Journal of Bacteriology, November 2007, p. 8353-8356, Vol. 189, No. 22
0021-9193/07/$08.00+0     doi:10.1128/JB.00620-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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