Previous Article | Next Article 
Journal of Bacteriology, November 2007, p. 8353-8356, Vol. 189, No. 22
0021-9193/07/$08.00+0 doi:10.1128/JB.00620-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.
Pseudomonas aeruginosa Psl Is a Galactose- and Mannose-Rich Exopolysaccharide
,
Luyan Ma,1
Haiping Lu,1
April Sprinkle,1
Matthew R. Parsek,2 and
Daniel J. Wozniak1*
Department of Microbiology and Immunology, Wake Forest University School of Medicine, Winston-Salem, North Carolina,1
Department of Microbiology, University of Washington, Seattle, Washington2
Received 21 April 2007/
Accepted 4 July 2007
The Pseudomonas aeruginosa polysaccharide synthesis locus (psl) is predicted to encode an exopolysaccharide which is critical for biofilm formation. Here we used chemical composition analyses and mannose- or galactose-specific lectin staining, followed by confocal laser scanning microscopy and electron microscopy, to show that Psl is a galactose-rich and mannose-rich exopolysaccharide.
* Corresponding author. Mailing address: Department of Microbiology and Immunology, Wake Forest University School of Medicine, Medical Center Blvd., Winston-Salem, NC 27157-1064. Phone: (336) 716-2016. Fax: (336) 716-9928. E-mail:
dwozniak{at}wfubmc.edu
Published ahead of print on 13 July 2007.
Supplemental material for this article may be found at http://jb.asm.org/.
Journal of Bacteriology, November 2007, p. 8353-8356, Vol. 189, No. 22
0021-9193/07/$08.00+0 doi:10.1128/JB.00620-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.
This article has been cited by other articles:
-
Karatan, E., Watnick, P.
(2009). Signals, Regulatory Networks, and Materials That Build and Break Bacterial Biofilms. Microbiol. Mol. Biol. Rev.
73: 310-347
[Abstract]
[Full Text]
-
Mikkelsen, H., Bond, N. J., Skindersoe, M. E., Givskov, M., Lilley, K. S., Welch, M.
(2009). Biofilms and type III secretion are not mutually exclusive in Pseudomonas aeruginosa. Microbiology
155: 687-698
[Abstract]
[Full Text]
-
Darnell, C. L., Hussa, E. A., Visick, K. L.
(2008). The Putative Hybrid Sensor Kinase SypF Coordinates Biofilm Formation in Vibrio fischeri by Acting Upstream of Two Response Regulators, SypG and VpsR. J. Bacteriol.
190: 4941-4950
[Abstract]
[Full Text]
-
Itoh, Y., Rice, J. D., Goller, C., Pannuri, A., Taylor, J., Meisner, J., Beveridge, T. J., Preston, J. F. III, Romeo, T.
(2008). Roles of pgaABCD Genes in Synthesis, Modification, and Export of the Escherichia coli Biofilm Adhesin Poly-{beta}-1,6-N-Acetyl-D-Glucosamine. J. Bacteriol.
190: 3670-3680
[Abstract]
[Full Text]