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Journal of Bacteriology, November 2007, p. 8361-8365, Vol. 189, No. 22
0021-9193/07/$08.00+0     doi:10.1128/JB.01028-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

The Escherichia coli Yej Transporter Is Required for the Uptake of Translation Inhibitor Microcin C{triangledown}

Maria Novikova,1 Anastasia Metlitskaya,1 Kirill Datsenko,2 Teymur Kazakov,1,3 Alexey Kazakov,4 Barry Wanner,2 and Konstantin Severinov1,3,5,6*

Institute of Molecular Genetics, Russian Academy of Sciences, Moscow 123182, Russia,1 Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907,2 Waksman Institute, Piscataway, New Jersey 08854,3 Institute for Information Transmission Problems, Russian Academy of Sciences, Moscow 127994, Russia,4 Department of Molecular Biology and Biochemistry, Rutgers, The State University, Piscataway, New Jersey 08854,5 Institute of Gene Biology, Russian Academy of Sciences, Moscow 117312, Russia6

Received 29 June 2007/ Accepted 28 August 2007

Microcin C (McC), a peptide-nucleotide antibiotic, targets aspartyl-tRNA synthetase. By analyzing a random transposon library, we identified Escherichia coli mutants resistant to McC. Transposon insertions were localized to a single locus, yejABEF, which encodes components of a putative inner membrane ABC transporter. Analysis of site-specific mutants established that all four components of the transporter are required for McC sensitivity. Since aspartyl-tRNA synthetase in yej mutant extracts was fully sensitive to McC, we conclude that yej mutations interfere with McC uptake and that YejABEF is the only inner membrane transporter responsible for McC uptake in E. coli. Other substrates of YejABEF remain to be identified.

* Corresponding author. Mailing address: Waksman Institute, 190 Frelinghuysen Road, Piscataway, NJ 08854. Phone: (732) 445-6095. Fax: (732) 445-5735. E-mail: severik{at}waksman.rutgers.edu

{triangledown} Published ahead of print on 14 September 2007.

Journal of Bacteriology, November 2007, p. 8361-8365, Vol. 189, No. 22
0021-9193/07/$08.00+0     doi:10.1128/JB.01028-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Metlitskaya, A., Kazakov, T., Vondenhoff, G. H., Novikova, M., Shashkov, A., Zatsepin, T., Semenova, E., Zaitseva, N., Ramensky, V., Van Aerschot, A., Severinov, K. (2009). Maturation of the Translation Inhibitor Microcin C. J. Bacteriol. 191: 2380-2387 [Abstract] [Full Text]  
  • Kazakov, T., Vondenhoff, G. H., Datsenko, K. A., Novikova, M., Metlitskaya, A., Wanner, B. L., Severinov, K. (2008). Escherichia coli Peptidase A, B, or N Can Process Translation Inhibitor Microcin C. J. Bacteriol. 190: 2607-2610 [Abstract] [Full Text]  


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