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Polymerization of the Actin-Like Protein MamK, Which Is Associated with Magnetosomes

Azuma Taoka,^1, Ryuji Asada,^1, Long-Fei Wu,² and Yoshihiro Fukumori^1*

Department of Life Science, Graduate School of Natural Science and Technology, Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan,¹ Laboratoire de Chimie Bactérienne, UPR9043, IBSM, CNRS, 31, Chemin Joseph Aiguier, 13402 Marseille cedex 20, France²

Received 8 June 2007/ Accepted 20 September 2007

The recombinant actin-like protein MamK was purified from Escherichia coli and used as an antigen to generate the anti-MamK antibody. Immunostaining studies showed a linear distribution of MamK in Magnetospirillum magnetotacticum cells and of MamK in association with magnetosomes. Moreover, we demonstrated that MamK polymerizes into filamentous bundles in vitro.

Published ahead of print on 28 September 2007.

A.T. and R.A. contributed equally to this work.