This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sezonov, G.
Right arrow Articles by D'Ari, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sezonov, G.
Right arrow Articles by D'Ari, R.

 Previous Article  |  Next Article 

Journal of Bacteriology, December 2007, p. 8746-8749, Vol. 189, No. 23
0021-9193/07/$08.00+0     doi:10.1128/JB.01368-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Escherichia coli Physiology in Luria-Bertani Broth{triangledown}

Guennadi Sezonov,* Danièle Joseleau-Petit, and Richard D'Ari

Institut Jacques Monod, CNRS, Université Paris 6, Université Paris 7, F-75251 Paris Cedex 05, France

Received 22 August 2007/ Accepted 14 September 2007

Luria-Bertani broth supports Escherichia coli growth to an optical density at 600 nm (OD600) of 7. Surprisingly, however, steady-state growth ceases at an OD600 of 0.3, when the growth rate slows down and cell mass decreases. Growth stops for lack of a utilizable carbon source. The carbon sources for E. coli in Luria-Bertani broth are catabolizable amino acids, not sugars.

* Corresponding author. Present address: Unité Biologie Moléculaire du Gène chez les Extrémophiles, Institut Pasteur, 25, rue du Docteur Roux, F-75724 Paris Cedex 15, France. Phone: 331 4061 3722. Fax: 331 4568 8834. E-mail: sezonov{at}pasteur.fr

{triangledown} Published ahead of print on 28 September 2007.

Journal of Bacteriology, December 2007, p. 8746-8749, Vol. 189, No. 23
0021-9193/07/$08.00+0     doi:10.1128/JB.01368-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Lopez, C. M., Rholl, D. A., Trunck, L. A., Schweizer, H. P. (2009). Versatile Dual-Technology System for Markerless Allele Replacement in Burkholderia pseudomallei. Appl. Environ. Microbiol. 75: 6496-6503 [Abstract] [Full Text]  
  • Ruhl, J., Schmid, A., Blank, L. M. (2009). Selected Pseudomonas putida Strains Able To Grow in the Presence of High Butanol Concentrations. Appl. Environ. Microbiol. 75: 4653-4656 [Abstract] [Full Text]  
  • Vilain, S., Pretorius, J. M., Theron, J., Brozel, V. S. (2009). DNA as an Adhesin: Bacillus cereus Requires Extracellular DNA To Form Biofilms. Appl. Environ. Microbiol. 75: 2861-2868 [Abstract] [Full Text]  
  • Rice, C. D., Pollard, J. E., Lewis, Z. T., McCleary, W. R. (2009). Employment of a Promoter-Swapping Technique Shows that PhoU Modulates the Activity of the PstSCAB2 ABC Transporter in Escherichia coli. Appl. Environ. Microbiol. 75: 573-582 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»