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Identification of Promoters Recognized by RNA Polymerase-^E Holoenzyme from Thermus thermophilus HB8 ^,

RIKEN SPring-8 Center, Harima Institute, 1-1-1 Kouto, Sayo, Hyogo 679-5148, Japan,¹ Genomic Sciences Center, Yokohama Institute, RIKEN, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan,² Department of Biological Sciences, Graduate School of Science, Osaka University, Toyonaka, Osaka 560-0043, Japan,³ Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan⁴

Received 7 July 2007/ Accepted 17 September 2007

Thermus thermophilus ^E, an extracytoplasmic function factor from the extremely thermophilic bacterium Thermus thermophilus HB8, bound to the RNA polymerase core enzyme and showed transcriptional activity. With the combination of in vitro transcription assay and GeneChip technology, we identified three promoters recognized by ^E. The predicted consensus promoter sequence for ^E is 5'-CA(A/T)(A/C)C(A/C)-N₁₅-CCGTA-3'.

Published ahead of print on 28 September 2007.

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