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Journal of Bacteriology, December 2007, p. 8855-8862, Vol. 189, No. 24
0021-9193/07/$08.00+0     doi:10.1128/JB.01213-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Cohabitation of Two Different lexA Regulons in Pseudomonas putida{triangledown} ,{dagger}

Marc Abella,1,{ddagger} Susana Campoy,2,{ddagger} Ivan Erill,3 Fernando Rojo,4 and Jordi Barbé1,2*

Departament de Genètica i Microbiologia, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain,1 Centre de Recerca en Sanitat Animal (CReSA), 08193 Bellaterra, Spain,2 Biomedical Applications Group, Centro Nacional de Microelectrónica, CNM-IMB (CSIC), 08193 Bellaterra, Spain,3 Departamento de Biotecnología Microbiana, Centro Nacional de Biotecnología, CSIC, Campus de la Universidad Autónoma de Madrid, Cantoblanco, 28049 Madrid, Spain4

Received 27 July 2007/ Accepted 1 October 2007

In contrast to the vast majority of the members of the domain Bacteria, several Pseudomonas and Xanthomonas species have two lexA genes, whose products have been shown to recognize different LexA binding motifs, making them an interesting target for studying the interplay between cohabiting LexA regulons in a single species. Here we report an analysis of the genetic composition of the two LexA regulons of Pseudomonas putida KT2440 performed with a genomic microarray. The data obtained indicate that one of the two LexA proteins (LexA1) seems to be in control of the conventional Escherichia coli-like SOS response, while the other LexA protein (LexA2) regulates only its own transcriptional unit, which includes the imuA, imuB, and dnaE2 genes, and a gene (PP_3901) from a resident P. putida prophage. Furthermore, PP_3901 is also regulated by LexA1 and is required for DNA damage-mediated induction of several P. putida resident prophage genes. In silico searches suggested that this marked asymmetry in regulon contents also occurs in other Pseudomonas species with two lexA genes, and the implications of this asymmetry in the evolution of the SOS network are discussed.

* Corresponding author. Mailing address: Departament de Genètica i Microbiologia, Ed. Cn, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain. Phone: 34-93-581 1837. Fax: 34-93 581 2387. E-mail: jordi.barbe{at}uab.es

{triangledown} Published ahead of print on 12 October 2007.

{dagger} Supplemental material for this article may be found at http://jb.asm.org/.

{ddagger} M. Abella and S. Campoy are joint first authors.

Journal of Bacteriology, December 2007, p. 8855-8862, Vol. 189, No. 24
0021-9193/07/$08.00+0     doi:10.1128/JB.01213-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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