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Journal of Bacteriology, December 2007, p. 9108-9116, Vol. 189, No. 24
0021-9193/07/$08.00+0     doi:10.1128/JB.00983-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Introduction of the Foreign Transposon Tn4560 in Streptomyces coelicolor Leads to Genetic Instability near the Native Insertion Sequence IS1649{triangledown} ,{dagger}

Elizabeth M. Widenbrant* and Camilla M. Kao

Department of Chemical Engineering, Stanford University, Stanford, California 94305

Received 20 June 2007/ Accepted 9 October 2007

We report an altered pattern of genetic instability for Streptomyces coelicolor when the bacterium harbored a foreign transposon, Tn4560. Deletions, amplifications, and circularizations of the linear 8.7-Mb chromosome occurred more frequently at sites adjacent to native insertion elements, notably IS1649. In contrast, deletions, amplifications, and circularizations of a wild-type strain happened at heterogeneous sites within the chromosome. In 50 strains examined, structural changes removed or duplicated hundreds of contiguous S. coelicolor genes, altering up to 33% of the chromosome. S. coelicolor shows a bias toward one type of genetic instability during this particular assault from the environment, the invasion of foreign DNA.

* Corresponding author. Mailing address: Department of Chemical Engineering, Stanford University, Stanford, CA 94305. Phone: (650) 723-4906. Fax: (650) 723-9780. E-mail: ewidenbrant{at}gmail.com

{triangledown} Published ahead of print on 19 October 2007.

{dagger} Supplemental material for this article may be found at http://jb.asm.org/.

Journal of Bacteriology, December 2007, p. 9108-9116, Vol. 189, No. 24
0021-9193/07/$08.00+0     doi:10.1128/JB.00983-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Widenbrant, E. M., Tsai, H.-H., Chen, C. W., Kao, C. M. (2008). Spontaneous Amplification of the Actinorhodin Gene Cluster in Streptomyces coelicolor Involving Native Insertion Sequence IS466. J. Bacteriol. 190: 4754-4758 [Abstract] [Full Text]  
  • Widenbrant, E. M., Tsai, H.-H., Chen, C. W., Kao, C. M. (2007). Streptomyces coelicolor Undergoes Spontaneous Chromosomal End Replacement. J. Bacteriol. 189: 9117-9121 [Abstract] [Full Text]  


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