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Journal of Bacteriology, February 2007, p. 1311-1321, Vol. 189, No. 4
0021-9193/07/$08.00+0     doi:10.1128/JB.01393-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Similarity and Differences in the Lactobacillus acidophilus Group Identified by Polyphasic Analysis and Comparative Genomics{triangledown} ,{dagger}

Bernard Berger,* R. David Pridmore, Caroline Barretto, Françoise Delmas-Julien, Kerstin Schreiber, Fabrizio Arigoni, and Harald Brüssow*

Nestlé Research Center, CH-1000 Lausanne 26, Vers-chez-les-Blanc, Switzerland

Received 31 August 2006/ Accepted 21 November 2006

A set of lactobacilli were investigated by polyphasic analysis. Multilocus sequence analysis, DNA typing, microarray analysis, and in silico whole-genome alignments provided a remarkably consistent pattern of similarity within the Lactobacillus acidophilus complex. On microarray analysis, 17 and 5% of the genes from Lactobacillus johnsonii strain NCC533 represented variable and strain-specific genes, respectively, when tested against four independent isolates of L. johnsonii. When projected on the NCC533 genome map, about 10 large clusters of variable genes were identified, and they were enriched around the terminus of replication. A quarter of the variable genes and two-thirds of the strain-specific genes were associated with mobile DNA. Signatures for horizontal gene transfer and modular evolution were found in prophages and in DNA from the exopolysaccharide biosynthesis cluster. On microarray hybridizations, Lactobacillus gasseri strains showed a shift to significantly lower fluorescence intensities than the L. johnsonii test strains, and only genes encoding very conserved cellular functions from L. acidophilus hybridized to the L. johnsonii array. In-silico comparative genomics showed extensive protein sequence similarity and genome synteny of L. johnsonii with L. gasseri, L. acidophilus, and Lactobacillus delbrueckii; moderate synteny with Lactobacillus casei; and scattered X-type sharing of protein sequence identity with the other sequenced lactobacilli. The observation of a stepwise decrease in similarity between the members of the L. acidophilus group suggests a strong element of vertical evolution in a natural phylogenetic group. Modern whole-genome-based techniques are thus a useful adjunct to the clarification of taxonomical relationships in problematic bacterial groups.

* Corresponding author. Mailing address: Nestlé Research Center, CH-1000 Lausanne 26, Vers-chez-les-Blanc, Switzerland. Phone for B. Berger: 41 21 785 8955. Phone for H. Brüssow: 41 21 785 8676. Fax: 41 21 785 8544. E-mail for B. Berger: bernard.berger{at}rdls.nestle.com. E-mail for H. Brüssow: fharald.bruessow{at}rdls.nestle.com.

{triangledown} Published ahead of print on 1 December 2006.

{dagger} Supplemental material for this article may be found at http://jb.asm.org/.

Journal of Bacteriology, February 2007, p. 1311-1321, Vol. 189, No. 4
0021-9193/07/$08.00+0     doi:10.1128/JB.01393-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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