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Journal of Bacteriology, February 2007, p. 1441-1450, Vol. 189, No. 4
0021-9193/07/$08.00+0     doi:10.1128/JB.00832-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Structure-Activity Analysis of Quorum-Sensing Signaling Peptides from Streptococcus mutans{triangledown} ,{dagger}

Raymond T. Syvitski,1 Xiao-Lin Tian,2 Kamal Sampara,2 Alan Salman,3 Song F. Lee,2,3 David L. Jakeman,1 and Yung-Hua Li2,3*

College of Pharmacy,1 Department of Applied Oral Sciences,2 Department of Microbiology and Immunology, Dalhousie University, Halifax, Nova Scotia, Canada3

Received 12 June 2006/ Accepted 18 August 2006

Streptococcus mutans secretes and utilizes a 21-amino-acid signaling peptide pheromone to initiate quorum sensing for genetic competence, biofilm formation, stress responses, and bacteriocin production. In this study, we designed and synthesized a series of truncated peptides and peptides with amino acid substitutions to investigate their structure-activity relationships based on the three-dimensional structures of S. mutans wild-type signaling peptide UA159sp and C-terminally truncated peptide TPC3 from mutant JH1005 defective in genetic competence. By analyzing these peptides, we demonstrated that the signaling peptide of S. mutans has at least two functional domains. The C-terminal structural motif consisting of a sequence of polar hydrophobic charged residues is crucial for activation of the signal transduction pathway, while the core {alpha}-helical structure extending from residue 5 to the end of the peptide is required for receptor binding. Peptides in which three or more residues were deleted from the C terminus did not induce genetic competence but competitively inhibited quorum sensing activated by UA159sp. Disruption of the amphipathic {alpha}-helix by replacing the Phe-7, Phe-11, or Phe-15 residue with a hydrophilic residue resulted in a significant reduction in or complete loss of the activity of the peptide. In contrast to the C-terminally truncated peptides, these peptides with amino acid substitutions did not compete with UA159sp to activate quorum sensing, suggesting that disruption of the hydrophobic face of the {alpha}-helical structure results in a peptide that is not able to bind to the receptor. This study is the first study to recognize the importance of the signaling peptide C-terminal residues in streptococcal quorum sensing.

* Corresponding author. Mailing address: 5981 University Ave., Rm5215, Halifax, Nova Scotia, Canada B3H 3J5. Phone: (902) 494-3063. Fax: (902) 494-6621. E-mail: yung-hua.li{at}dal.ca.

{triangledown} Published ahead of print on 25 August 2006.

{dagger} Supplemental material for this article may be found at http://jb.asm.org/.

Journal of Bacteriology, February 2007, p. 1441-1450, Vol. 189, No. 4
0021-9193/07/$08.00+0     doi:10.1128/JB.00832-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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