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A Suppressor of Cell Death Caused by the Loss of ^E Downregulates Extracytoplasmic Stress Responses and Outer Membrane Vesicle Production in Escherichia coli

Julie E. Button, Thomas J. Silhavy, and Natividad Ruiz^*

Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544

Received 2 October 2006/ Accepted 8 December 2006

When envelope biogenesis is compromised or damage to envelope components occurs, bacteria trigger signaling cascades, which lead to the production of proteins that combat such extracytoplasmic stresses. In Escherichia coli, there are three pathways known to deal with envelope stresses: the Bae, Cpx, and ^E responses. Although the effectors of the Bae and Cpx responses are not essential in E. coli, the effector of the ^E response, the sigma factor RpoE (^E), is essential for viability. However, mutations that suppress the lethality of an rpoE-null allele can be easily obtained, and here we describe how we have isolated at least four classes of these suppressors. We present the first description of one such suppressor class, loss-of-function mutations in ydcQ, a gene encoding a putative DNA-binding protein. In wild-type rpoE⁺ strains, ydcQ mutants have two distinct phenotypes: extracytoplasmic stress responses are significantly downregulated, and the production of outer membrane vesicles is severely reduced. We present a model in which ^E is not essential per se but, rather, we propose that rpoE mutant cells die, possibly because they overreact to the absence of this factor by triggering a cell death signal.

Published ahead of print on 15 December 2006.

Present address: Boyer Center for Molecular Medicine, Yale University School of Medicine, 295 Congress Ave., Rm. 331, New Haven, CT 06536.

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