This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Guisbert, E.
Right arrow Articles by Gross, C. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Guisbert, E.
Right arrow Articles by Gross, C. A.

 Previous Article  |  Next Article 

Journal of Bacteriology, March 2007, p. 1963-1973, Vol. 189, No. 5
0021-9193/07/$08.00+0     doi:10.1128/JB.01243-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Hfq Modulates the {sigma}E-Mediated Envelope Stress Response and the {sigma}32-Mediated Cytoplasmic Stress Response in Escherichia coli{triangledown} ,{ddagger}

Eric Guisbert ,1,{dagger},§ Virgil A. Rhodius,2,{dagger} Nidhi Ahuja,2 Emily Witkin,2 and Carol A. Gross2,3*

Departments of Biochemistry and Biophysics,1 Microbiology and Immunology,2 Cell and Tissue Biology, University of California, San Francisco, California3

Received 8 August 2006/ Accepted 1 December 2006

Hfq, a chaperone for small noncoding RNAs, regulates many processes in Escherichia coli, including the {sigma}S-mediated general stress response. Here we used microarray analysis to identify the changes in gene expression resulting from lack of Hfq. We identify several potential new targets for Hfq regulation, including genes encoding outer membrane proteins, enzymes, factors, and transporters. Many of these genes are involved in amino acid uptake and biosynthesis, sugar uptake and metabolism, and cell energetics. In addition, we find altered regulation of the {sigma}E- and {sigma}32-mediated stress responses, which we analyze further. We show that cells lacking Hfq induce the {sigma}E-mediated envelope stress response and are defective in {sigma}E-mediated repression of outer membrane proteins. We also show that the {sigma}32-mediated cytoplasmic stress response is repressed in cells lacking Hfq due to increased expression of DnaK. Furthermore, we show that cells lacking Hfq are defective in the "long-term adaptation" of {sigma}32 to chronic chaperone overexpression. Together, our results indicate that Hfq may play a general role in stress response regulation in E. coli.

* Corresponding author. Mailing address: Genentech Hall, Rm. S376, 600 16th St., University of California at San Francisco, San Francisco, CA 94158. Phone: (415) 476-4161. Fax: (415) 514-4080. E-mail: cgross{at}cgl.ucsf.edu.

{triangledown} Published ahead of print on 8 December 2006.

{ddagger} Supplemental material for this article may be found at http://jb.asm.org/.

{dagger} E.G. and V.A.R. contributed equally to this study.

§ Present address: Department of Biochemistry, Molecular Biology, and Cell Biology, Northwestern University, Chicago, Ill.

Journal of Bacteriology, March 2007, p. 1963-1973, Vol. 189, No. 5
0021-9193/07/$08.00+0     doi:10.1128/JB.01243-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Figueroa-Bossi, N., Valentini, M., Malleret, L., Bossi, L. (2009). Caught at its own game: regulatory small RNA inactivated by an inducible transcript mimicking its target. Genes Dev. 23: 2004-2015 [Abstract] [Full Text]  
  • Fantappie, L., Metruccio, M. M. E., Seib, K. L., Oriente, F., Cartocci, E., Ferlicca, F., Giuliani, M. M., Scarlato, V., Delany, I. (2009). The RNA Chaperone Hfq Is Involved in Stress Response and Virulence in Neisseria meningitidis and Is a Pleiotropic Regulator of Protein Expression. Infect. Immun. 77: 1842-1853 [Abstract] [Full Text]  
  • Meibom, K. L., Forslund, A.-L., Kuoppa, K., Alkhuder, K., Dubail, I., Dupuis, M., Forsberg, A., Charbit, A. (2009). Hfq, a Novel Pleiotropic Regulator of Virulence-Associated Genes in Francisella tularensis. Infect. Immun. 77: 1866-1880 [Abstract] [Full Text]  
  • Price, N. L., Raivio, T. L. (2009). Characterization of the Cpx Regulon in Escherichia coli Strain MC4100. J. Bacteriol. 191: 1798-1815 [Abstract] [Full Text]  
  • De Lay, N., Gottesman, S. (2009). The Crp-Activated Small Noncoding Regulatory RNA CyaR (RyeE) Links Nutritional Status to Group Behavior. J. Bacteriol. 191: 461-476 [Abstract] [Full Text]  
  • Pulvermacher, S. C., Stauffer, L. T., Stauffer, G. V. (2009). Role of the Escherichia coli Hfq protein in GcvB regulation of oppA and dppA mRNAs. Microbiology 155: 115-123 [Abstract] [Full Text]  
  • Pulvermacher, S. C., Stauffer, L. T., Stauffer, G. V. (2009). The Small RNA GcvB Regulates sstT mRNA Expression in Escherichia coli. J. Bacteriol. 191: 238-248 [Abstract] [Full Text]  
  • Gorke, B., Vogel, J. (2008). Noncoding RNA control of the making and breaking of sugars. Genes Dev. 22: 2914-2925 [Abstract] [Full Text]  
  • Guisbert, E., Yura, T., Rhodius, V. A., Gross, C. A. (2008). Convergence of Molecular, Modeling, and Systems Approaches for an Understanding of the Escherichia coli Heat Shock Response. Microbiol. Mol. Biol. Rev. 72: 545-554 [Abstract] [Full Text]  
  • Sharma, C. M., Darfeuille, F., Plantinga, T. H., Vogel, J. (2007). A small RNA regulates multiple ABC transporter mRNAs by targeting C/A-rich elements inside and upstream of ribosome-binding sites. Genes Dev. 21: 2804-2817 [Abstract] [Full Text]  
  • Wilson, J. W., Ott, C. M., zu Bentrup, K. H., Ramamurthy, R., Quick, L., Porwollik, S., Cheng, P., McClelland, M., Tsaprailis, G., Radabaugh, T., Hunt, A., Fernandez, D., Richter, E., Shah, M., Kilcoyne, M., Joshi, L., Nelman-Gonzalez, M., Hing, S., Parra, M., Dumars, P., Norwood, K., Bober, R., Devich, J., Ruggles, A., Goulart, C., Rupert, M., Stodieck, L., Stafford, P., Catella, L., Schurr, M. J., Buchanan, K., Morici, L., McCracken, J., Allen, P., Baker-Coleman, C., Hammond, T., Vogel, J., Nelson, R., Pierson, D. L., Stefanyshyn-Piper, H. M., Nickerson, C. A. (2007). Space flight alters bacterial gene expression and virulence and reveals a role for global regulator Hfq. Proc. Natl. Acad. Sci. USA 104: 16299-16304 [Abstract] [Full Text]  
  • Sklar, J. G., Wu, T., Kahne, D., Silhavy, T. J. (2007). Defining the roles of the periplasmic chaperones SurA, Skp, and DegP in Escherichia coli. Genes Dev. 21: 2473-2484 [Abstract] [Full Text]  
  • Thompson, K. M., Rhodius, V. A., Gottesman, S. (2007). {sigma}E Regulates and Is Regulated by a Small RNA in Escherichia coli. J. Bacteriol. 189: 4243-4256 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»