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Journal of Bacteriology, March 2007, p. 2055-2062, Vol. 189, No. 5
0021-9193/07/$08.00+0     doi:10.1128/JB.01331-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

hpaR, a Putative marR Family Transcriptional Regulator, Is Positively Controlled by HrpG and HrpX and Involved in the Pathogenesis, Hypersensitive Response, and Extracellular Protease Production of Xanthomonas campestris Pathovar campestris{triangledown}

Ke Wei,1,2,{dagger} Dong-Jie Tang,2,{dagger} Yong-Qiang He,2 Jia-Xun Feng,2 Bo-Le Jiang,2 Guang-Tao Lu,2 Baoshan Chen,2 and Ji-Liang Tang2*

College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029, China,1 Guangxi Key Laboratory of Subtropical Bioresources Conservation and Utilization, The Key Laboratory of Ministry of Education for Microbial and Plant Genetic Engineering, and College of Life Science and Technology, Guangxi University, 100 Daxue Road, Nanning Guangxi 530004, China2

Received 21 August 2006/ Accepted 2 December 2006

The MarR family of transcriptional regulators of bacteria are involved in the regulation of many cellular processes, including pathogenesis. In this work, we have demonstrated genetically that hpaR (hpa, hrp associated), which encodes a putative MarR family regulator, is involved in the hypersensitive response (HR), pathogenicity, and extracellular protease production of the phytopathogenic bacterium Xanthomonas campestris pathovar campestris. A mutation in hpaR resulted in complete loss of virulence in the host plant cabbage, a delayed and weakened HR in the nonhost plant pepper ECW-10R, and an increase in extracellular protease production. Detection of the ß-glucuronidase activity of a plasmid-driven hpaR promoter-gusA reporter revealed that the expression of hpaR is positively controlled by HrpG and HrpX and is suppressed in rich medium while being strongly induced in minimal and hrp-inducing media and inside the host. These findings indicate that hpaR belongs to the hrpG and hrpX regulon and that HrpX regulates the extracellular protease production via hpaR in X. campestris pv. campestris.

* Corresponding author. Mailing address: College of Life Science and Technology, Guangxi University, 100 Daxue Road, Nanning, Guangxi 530004, China. Phone: 86-771-3239566. Fax: 86-771-3239413. E-mail: jltang{at}gxu.edu.cn.

{triangledown} Published ahead of print on 8 December 2006.

{dagger} These authors contributed equally to this work.

Journal of Bacteriology, March 2007, p. 2055-2062, Vol. 189, No. 5
0021-9193/07/$08.00+0     doi:10.1128/JB.01331-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

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