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Journal of Bacteriology, March 2007, p. 2114-2118, Vol. 189, No. 5
0021-9193/07/$08.00+0     doi:10.1128/JB.01609-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Amino Acid Residues Required for Maturation, Cell Uptake, and Processing of Translation Inhibitor Microcin C{triangledown}

Teymur Kazakov,1,2 Anastasia Metlitskaya,3 and Konstantin Severinov1,3,4*

Waksman Institute, Piscataway, New Jersey 08854,1 Institute of Protein Research, Russian Academy of Sciences, Puschino, 142292 Russia,2 Institute of Molecular Genetics, Russian Academy of Sciences, Moscow, 123182 Russia,3 Department of Molecular Biology and Biochemistry, Rutgers, The State University, Piscataway, New Jersey 088544

Received 16 October 2006/ Accepted 29 November 2006

Microcin C (McC), a peptide-nucleotide Trojan horse antibiotic, targets aspartyl-tRNA synthetase. We present the results of a systematic mutational study of the 7-amino-acid ribosomally synthesized peptide moiety of McC. Our results define amino acid positions important for McC maturation and cell uptake and processing and open the way for creation of more potent McC-based inhibitors.

* Corresponding author. Mailing address: Waksman Institute, 190 Frelinghuysen Road, Piscataway, NJ 08854. Phone: (732) 445-6095. Fax: (732) 445-5735. E-mail: severik{at}waksman.rutgers.edu.

{triangledown} Published ahead of print on 8 December 2006.

Journal of Bacteriology, March 2007, p. 2114-2118, Vol. 189, No. 5
0021-9193/07/$08.00+0     doi:10.1128/JB.01609-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Van de Vijver, P., Vondenhoff, G. H. M., Kazakov, T. S., Semenova, E., Kuznedelov, K., Metlitskaya, A., Van Aerschot, A., Severinov, K. (2009). Synthetic Microcin C Analogs Targeting Different Aminoacyl-tRNA Synthetases. J. Bacteriol. 191: 6273-6280 [Abstract] [Full Text]  
  • Metlitskaya, A., Kazakov, T., Vondenhoff, G. H., Novikova, M., Shashkov, A., Zatsepin, T., Semenova, E., Zaitseva, N., Ramensky, V., Van Aerschot, A., Severinov, K. (2009). Maturation of the Translation Inhibitor Microcin C. J. Bacteriol. 191: 2380-2387 [Abstract] [Full Text]  
  • Kazakov, T., Vondenhoff, G. H., Datsenko, K. A., Novikova, M., Metlitskaya, A., Wanner, B. L., Severinov, K. (2008). Escherichia coli Peptidase A, B, or N Can Process Translation Inhibitor Microcin C. J. Bacteriol. 190: 2607-2610 [Abstract] [Full Text]  


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