This Article Full Text Full Text (PDF) Other Versions of this Article: JB.01247-06v1 189/5/2160    most recent Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Trainer, M. A. Articles by Kahn, M. L. Search for Related Content PubMed PubMed Citation Articles by Trainer, M. A. Articles by Kahn, M. L.

Role of a Conserved Membrane Glycine Residue in a Dicarboxylate Transporter from Sinorhizobium meliloti

Maria A. Trainer,^1,2, Svetlana N. Yurgel,¹ and Michael L. Kahn^1,2*

Institute of Biological Chemistry, Washington State University, Pullman, Washington 99164-6340,¹ School of Molecular Biosciences, Washington State University, Pullman, Washington 99164-6340²

Received 8 August 2006/ Accepted 28 November 2006

Nitrogen-fixing rhizobial bacteroids import dicarboxylates by using the DctA transporter. G114 of DctA is highly conserved. A G114D mutant is inactive, but DctA with a small amino acid (G114A) or a helix disrupter (G114P) retains significant activity. G114 probably interacts with other membrane helices in stabilizing a substrate-binding pocket.

Published ahead of print on 8 December 2006.

Present address: Department of Biology, University of Waterloo, 200 University Ave. W., Waterloo, ON N2L 3G1, Canada.