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Journal of Bacteriology, March 2007, p. 2160-2163, Vol. 189, No. 5
0021-9193/07/$08.00+0     doi:10.1128/JB.01247-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Role of a Conserved Membrane Glycine Residue in a Dicarboxylate Transporter from Sinorhizobium meliloti{triangledown}

Maria A. Trainer,1,2,{dagger} Svetlana N. Yurgel,1 and Michael L. Kahn1,2*

Institute of Biological Chemistry, Washington State University, Pullman, Washington 99164-6340,1 School of Molecular Biosciences, Washington State University, Pullman, Washington 99164-63402

Received 8 August 2006/ Accepted 28 November 2006

Nitrogen-fixing rhizobial bacteroids import dicarboxylates by using the DctA transporter. G114 of DctA is highly conserved. A G114D mutant is inactive, but DctA with a small amino acid (G114A) or a helix disrupter (G114P) retains significant activity. G114 probably interacts with other membrane helices in stabilizing a substrate-binding pocket.

* Corresponding author. Mailing address: Institute of Biological Chemistry, Washington State University, Pullman, WA 99164-6340. Phone: (509) 335-8327. Fax: (509) 335-7643. E-mail: kahn{at}wsu.edu.

{triangledown} Published ahead of print on 8 December 2006.

{dagger} Present address: Department of Biology, University of Waterloo, 200 University Ave. W., Waterloo, ON N2L 3G1, Canada.

Journal of Bacteriology, March 2007, p. 2160-2163, Vol. 189, No. 5
0021-9193/07/$08.00+0     doi:10.1128/JB.01247-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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