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Journal of Bacteriology, March 2007, p. 2174-2175, Vol. 189, No. 5
0021-9193/07/$08.00+0     doi:10.1128/JB.01462-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

"Cold-Sensitive" Mutants of the Lac Repressor{triangledown}

Andrew Barker,1,2 Stefan Oehler,1,3* and Benno Müller-Hill1

Institut für Genetik der Universität zu Köln, Zülpicherstr. 47, D-50674 Köln, Germany,1 Western Australian Institute for Medical Research, Rear 50 Murray Street, Perth, WA 6000, Australia,2 IMBB-FORTH, Vassilika Vouton, P.O. Box 1378, GR-71110 Heraklion, Crete, Greece3

Received 15 September 2006/ Accepted 6 December 2006

Thirteen of more than 4,000 single-amino-acid-replacement mutants of the Lac repressor, generated by suppression of amber nonsense mutants, were characterized as having a cold-sensitive phenotype. However, when expressed as missense mutations, none of the replacements cause cold sensitivity, implicating the suppression mechanism as being responsible for this phenotype.

* Corresponding author. Mailing address: IMBB-FORTH, Vassilika Vouton, P.O. Box 1378, GR-71110 Heraklion, Crete, Greece. Phone: 30 2810-391144. Fax: 30 2810-391104. E-mail: oehler{at}imbb.forth.gr.

{triangledown} Published ahead of print on 15 December 2006.

Journal of Bacteriology, March 2007, p. 2174-2175, Vol. 189, No. 5
0021-9193/07/$08.00+0     doi:10.1128/JB.01462-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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