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"Cold-Sensitive" Mutants of the Lac Repressor

Institut für Genetik der Universität zu Köln, Zülpicherstr. 47, D-50674 Köln, Germany,¹ Western Australian Institute for Medical Research, Rear 50 Murray Street, Perth, WA 6000, Australia,² IMBB-FORTH, Vassilika Vouton, P.O. Box 1378, GR-71110 Heraklion, Crete, Greece³

Received 15 September 2006/ Accepted 6 December 2006

Thirteen of more than 4,000 single-amino-acid-replacement mutants of the Lac repressor, generated by suppression of amber nonsense mutants, were characterized as having a cold-sensitive phenotype. However, when expressed as missense mutations, none of the replacements cause cold sensitivity, implicating the suppression mechanism as being responsible for this phenotype.

Published ahead of print on 15 December 2006.